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Kif1bβ-pcDNA3

Plasmid

#22534

Depositor

William Kaelin

Article

Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

Insert

Kif1bβ (KIF1B Human)

Tags

FLAG

Expression

Mammalian

Mutation

wt cDNA

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pTRE3G-GSX2

Plasmid

#96964

Purpose

Dox-inducible expression of Gsx2 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 (Gsx2 Mouse)

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pU6_rd6_pegRNA_(PP7-C4-Q1)

Plasmid

#232433

Purpose

pegRNA with optimized 3' modifications to correct the rd6 mutation

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-2

Plasmid

#161819

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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GPS-MYC

Plasmid

#160130

Purpose

A reporter that monitors MYC protein expression.

Depositor

Channing Der

Article

Blake et al Sci Signal. 2019 Jul 16;12(590). pii: 12

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-eGFP

Plasmid

#164519

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG3_M18

Plasmid

#82356

Purpose

Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

Insert

mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

hEF1-HTLV prom

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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Epac-S-H326

Plasmid

#228242

Purpose

mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

Insert

mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Epac-S-H332

Plasmid

#228250

Purpose

mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

Insert

mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_HEK3_nsgRNA(PP7)

Plasmid

#232444

Purpose

nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin S14C

Plasmid

#60614

Purpose

Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

S14C

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pHIV-BRAFV600E-mOrange2

Plasmid

#110732

Purpose

Mammalian Expression of BRAFV600E IRES H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRAFV600E IRES H2B-mOrange2 (BRAF Human)

Use

Lentiviral

Expression

Mammalian

Mutation

BRAF V600E

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron

Plasmid

#246435

Purpose

Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.

Depositor

Ailong Ke

Article

Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

Inserts

O.gue IscB with dead mutations (D60A, H269A) and delta-TID
EGFP

Tags

SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

Expression

Mammalian

Mutation

changed Aspartic Acid 60 to Alanine, changed Hist…

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pTetOne-human cGAS-HA

Plasmid

#186896

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304 ERG

Plasmid

#118625

Purpose

Overexpresses ERG protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (33-479) (ERG Human)

Use

Lentiviral

Tags

None and none

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-C91G

Plasmid

#125845

Purpose

express mutant BAP1-C91G

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

C91G

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 Y254C

Plasmid

#231587

Purpose

ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed tyrosine 254 to cysteine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P2-MDM2 (17-125)

Plasmid

#62063

Purpose

Expression of human MDM2 (17-125) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

MDM2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains residues 17-125

Promoter

tac

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mM_M18

Plasmid

#91729

Purpose

Expression plasmid coding for heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

Insert

immunoglobulin heavy constant mu (Ighm Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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