We narrowed to 8,835 results for: sgRNA

1. hNTa1-qgRNA-pYJA5

   Plasmid

   #217779

   Purpose

   Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROF441

   Plasmid

   #155336

   Purpose

   Binary vector for expression of a gRNA targeting AtAP1 promoter 

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Reckleen_3_multieditdropout

   Plasmid

   #233458

   Purpose

   Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

   Depositor

   Anke Becker

   Article

   Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

   Insert

   Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4
   

   Use

   CRISPR

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. UCK2_Deletion_gRNA_1

   Plasmid

   #204672

   Purpose

   Dual gRNA plasmid for UCK2 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   uridine-cytidine kinase 2 (UCK2 Human)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. UCK2_Deletion_gRNA_2

   Plasmid

   #204673

   Purpose

   Dual gRNA plasmid for UCK2 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   uridine-cytidine kinase 2 (UCK2 Human)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-U6-CNCB_sgPOU5F1

   Plasmid

   #242174

   Purpose

   A piggybac-based vector containing mouse U6 promoter-driven POU5F1sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   mouse U6 promoter

   Available Since

   Sept. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Solo-mStayGold-MAP4

   Plasmid

   #227296

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(AAVS1-1)-PGKpuroBFP-W

   Plasmid

   #211954

   Purpose

   Express safe-cutter control gRNA with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   safe-cutter control sgRNA_AAVS1-1 (AAVS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(AAVS1-2)-PGKpuroBFP-W

   Plasmid

   #211955

   Purpose

   Express safe-cutter control gRNA with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   safe-cutter control sgRNA_AAVS1-2 (AAVS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(CTRL00509)-PGKpuroBFP-W

    Plasmid

    #211956

    Purpose

    Express non-cutter control gRNA with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    non-cutter control sgRNA_CTRL00509
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(CTRL00626)-PGKpuroBFP-W

    Plasmid

    #211957

    Purpose

    Express non-cutter control gRNA with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    non-cutter control sgRNA_CTRL00626
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPtGE34

    Plasmid

    #107932

    Purpose

    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Phage-PGK-Flag-HA-FKBP-T(WT)

    Plasmid

    #181734

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Article

    Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. qTAG-C-Solo-mStayGold-COX8A

    Plasmid

    #227309

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid

    #217662

    Purpose

    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO5-SgHottip-GFP-CRISPRa

    Plasmid

    #134990

    Purpose

    dCas9-mediated activation of HOTTIP in mammalian cells

    Depositor

    Suming Huang

    Article

    Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

    Insert

    HOTTIP (HOTTIP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LCV2_mClover3_LacZ

    Plasmid

    #155103

    Purpose

    lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO5-SgHottip-GFP-CRISPRi

    Plasmid

    #134989

    Purpose

    dCas9-mediated inactivation of HOTTIP in mammalian cells

    Depositor

    Suming Huang

    Article

    Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

    Insert

    HOTTIP (HOTTIP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Phage-PGK-Flag-HA-FKBP-T(G177D)

    Plasmid

    #181735

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Article

    Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

    Plasmid

    #211760

    Purpose

    Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

    Depositor

    Glenn Yiu

    Article

    Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    gRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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