-
Plasmid#35151DepositorInsertAtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
UseSynthetic BiologyTagsExpressionBacterialMutationR364S in Mevalonate KinasePromoterLacUV5,Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHDE-35S-Cas9-mCherry
Plasmid#78931PurposeFor CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plantsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gβγ-BERKY3
Plasmid#158426PurposeBRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK3ct
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-KB-1753-Nluc
Plasmid#158603PurposePlasmid expressing KB-1753-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gαq*-BERKY3
Plasmid#158425PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
UseTags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gαi*-BERKY3
Plasmid#158424PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsmycExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ154
Plasmid#69305PurposeGateway entry vector with AteCas9 (Arabidopsis codon optimized)DepositorInsertAteCas9 (Arabidopsis codon optimized)
UseCRISPRTagsExpressionMutationNLS fusionPromoterAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUB-cHyPer2
Plasmid#84728PurposeExpresses HyPer2 in plant cells-biosensor for Hydrogen peroxideDepositorInsertHyPer2
UseTagsExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGem-nHyPer2:sHyper2
Plasmid#84737PurposeSimultaneous expresion of nuclear and stromal HyPer2 in plant cellsDepositorInsertHyPer2 targeted to nucleus
UseTagsNLS-SV40ExpressionPlantMutationPromoterpFMV/p35SAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only