We narrowed to 905 results for: pgem

1. pOpLacI:mini35ss (GB0534)

   Plasmid

   #68179

   Purpose

   Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   6xOpLacI:mini35S
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUPD_OsU3 (GB1184)

   Plasmid

   #68209

   Purpose

   Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   OsU3 promoter
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPAtUbq10 (GB0223)

   Plasmid

   #68174

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq10 promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPAct2noUTR (GB0192)

   Plasmid

   #68177

   Purpose

   Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtAct2 promoter (no UTR)
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHcPro (GB0067)

   Plasmid

   #68196

   Purpose

   Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   TEV HcPro coding region
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTAtUbq3 (GB0219)

   Plasmid

   #68192

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq3 terminator
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. BtA3N

   Plasmid

   #238981

   Purpose

   In vitro transcription of bovine arrestin-3 short isoform

   Depositor

   Vsevolod Gurevich

   Article

   Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

   Insert

   arrestin-3 short (ARR3 Bovine)
   

   Use

   In vitro transcription

   Mutation

   silent, Cla I side introduced before stop codon

   Promoter

   SP6

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTAct2 (GB0210)

   Plasmid

   #68191

   Purpose

   Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtAct2 terminator
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPMtb (GB0080)

   Plasmid

   #68167

   Purpose

   Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   SlMtb promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPTctp (GB0083)

    Plasmid

    #68169

    Purpose

    Provides the S. Lycopersicum translationally-controlled tumor protein homolog promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlTCTP promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5'UTRAct2 (GB0193)

    Plasmid

    #68178

    Purpose

    Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    5'UTR AtAct2
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMyb12 (GB0075)

    Plasmid

    #68199

    Purpose

    Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Myb12 coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPAt2S3 (GB0029)

    Plasmid

    #68162

    Purpose

    Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    At2S3 promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUPD_Mini35s(-60,UTR) (GB0050)

    Plasmid

    #68176

    Purpose

    Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    CaMV Mini35S promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH3mCHSH2

    Plasmid

    #101053

    Purpose

    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

    Depositor

    Jennifer Lodge

    Article

    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

    Insert

    Cryptococcus mCherry expression plasmid
    

    Expression

    Yeast

    Promoter

    Cryptococcus Histone3 promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. phumanCas9 (GB0575)

    Plasmid

    #68206

    Purpose

    Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTNos:BASTA:PNos (GB0023)

    Plasmid

    #68211

    Purpose

    Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    bar gene
    

    Use

    Unspecified

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    PNos

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLacIBD::SDRX (GB0673)

    Plasmid

    #68183

    Purpose

    Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    LacI Binding Domain fused to SRDX Transcriptional Repressor
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BtA3CL

    Plasmid

    #238992

    Purpose

    In vitro transcription of mutant bovine arrestin-3 short isoform

    Depositor

    Vsevolod Gurevich

    Article

    Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

    Insert

    arrestin-3 short (ARR3 Bovine)
    

    Use

    In vitro transcription

    Mutation

    C17S, C60 V, C126S, C141L, C151V, C243V, C252V, C…

    Promoter

    SP6

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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