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BRD3 gRNA (BRDN0001146442)

Plasmid

#76092

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD3 (BRD3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CD2 gRNA (BRDN0001148522)

Plasmid

#75828

Purpose

3rd generation lentiviral gRNA plasmid targeting human CD2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CD2 (CD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ARAF gRNA (BRDN0001148242)

Plasmid

#75661

Purpose

3rd generation lentiviral gRNA plasmid targeting human ARAF

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ARAF (ARAF Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHA1 gRNA (BRDN0001145712)

Plasmid

#75589

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHA1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHA1 (EPHA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIB2 gRNA (BRDN0001148332)

Plasmid

#75604

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB2 (TRIB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DYRK3 gRNA (BRDN0001145918)

Plasmid

#75547

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK3 (DYRK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA5 gRNA (BRDN0001146427)

Plasmid

#77373

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA5

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA5 (RPS6KA5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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FYN gRNA (BRDN0001145913)

Plasmid

#77245

Purpose

3rd generation lentiviral gRNA plasmid targeting human FYN

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FYN (FYN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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GNE gRNA (BRDN0001145858)

Plasmid

#77830

Purpose

3rd generation lentiviral gRNA plasmid targeting human GNE

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GNE (GNE Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A S974A

Plasmid

#69814

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Publication

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-B-838;+80pCalb2

Plasmid

#66750

Purpose

luciferase reporter for Calb2 promoter (-838 to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Publication

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Calb2 promoter (-838bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCNPpo6-H98A

Plasmid

#46087

Depositor

Raymond Monnat

Publication

Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PpoI H98A

Tags

NLS

Expression

Mammalian

Mutation

catalytically inactive H98A variant

Promoter

CMV

Available since

June 21, 2014

Availability

Academic Institutions and Nonprofits only

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p6611 MSCV-IP N-HAonly UBOX5

Plasmid

#35010

Depositor

Peter Howley

Publication

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

UBOX5 (Ubox5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
p173 Dync1i2.A (Ex1b)

Plasmid

#26443

Depositor

Elizabeth Fisher

Publication

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform A (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p176 Dync1i2.B (Ex1b)

Plasmid

#26444

Depositor

Elizabeth Fisher

Publication

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform B (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p197 Dync1i2.B (Ex1a)

Plasmid

#26447

Depositor

Elizabeth Fisher

Publication

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform B (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
PTRF/Cavin1_L (OZ513)

Plasmid

#27188

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)

Use

Zebrafish targeting

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
pTag5A-rPrmpMyc

Plasmid

#22734

Depositor

Lynn Hudson

Publication

Nielsen et al J Neurosci. 2006 Sep 27. 26(39):9881-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Proline-rich transmembrane protein

Tags

Myc

Expression

Mammalian

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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V2rf2-IRES-BL Targeting Vector

Plasmid

#15860

Depositor

Peter Mombaerts

Publication

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

V2rf2 (Vmn2r81 Mouse)

Expression

Bacterial

Available since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
XE132 XFz7-CS2P+

Plasmid

#16792

Depositor

Randall Moon

Publication

Brown et al Dev Growth Differ. 2000 Aug. 42(4):347-5

Insert

XFz7-CS2P+ (fzd7.L Frog)

Use

Xenopus expression

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only