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pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

Plasmid

#74263

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.R404X truncation

Promoter

CMV

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
LRRK2 gRNA (BRDN0001146754)

Plasmid

#77330

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRRK2 (LRRK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TAOK3 gRNA (BRDN0001148268)

Plasmid

#76736

Purpose

3rd generation lentiviral gRNA plasmid targeting human TAOK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TAOK3 (TAOK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK2A1 gRNA (BRDN0001146321)

Plasmid

#76675

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK2A1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK2A1 (CSNK2A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K3 gRNA (BRDN0001146677)

Plasmid

#76380

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K3 (MAP2K3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NEK1 gRNA (BRDN0001146251)

Plasmid

#76125

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK1 (NEK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERN2 gRNA (BRDN0001145316)

Plasmid

#75845

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERN2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERN2 (ERN2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IRAK4 gRNA (BRDN0001147550)

Plasmid

#75665

Purpose

3rd generation lentiviral gRNA plasmid targeting human IRAK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IRAK4 (IRAK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PBK gRNA (BRDN0001145450)

Plasmid

#75572

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PBK (PBK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEG03

Plasmid

#73841

Purpose

Encodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.

Depositor

Junghae Suh, Jeffrey Tabor

Article

Gomez et al ACS Nano. 2015 Nov 30.

Insert

PhyB(1-917) with C-term his tag (PIL2 Mustard Weed)

Use

Dictyostelium discoideum expression

Tags

6x his tag

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#67608

Purpose

bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

catalytic mutant LDIG(182-185)AAAA

Promoter

tac

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-N/pIRES puro Glue (M1-A497)

Plasmid

#73454

Purpose

Express human N-terminal part (M1-A497) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, Ha tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-tetO-Mek1-shRNA

Plasmid

#53179

Purpose

Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (Map2k1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-hINO80N1 (1-262)

Plasmid

#29441

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-262 (a.a.)

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
CACNA1D (2of2) or cav1.3b_L (OZ547)

Plasmid

#28072

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting CACNA1D (2of2) or cav1.3b (cacna1db Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
CACNA1D (2of2) or cav1.3b_R (OZ548)

Plasmid

#28073

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting CACNA1D (2of2) or cav1.3b (cacna1db Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
ldlr (low density lipoprotein receptor)_R (OZ540)

Plasmid

#27217

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting ldlr (LOC100329978 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
fox1 (a2bp1l)_R (OZ520)

Plasmid

#27195

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
ldlr (low density lipoprotein receptor)_L (OZ539)

Plasmid

#27216

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting ldlr (LOC100329978 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
fox1 (a2bp1l)_L (OZ519)

Plasmid

#27194

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only