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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,271 results for: LEA

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Showing: 8841 - 8860 of 16271 results

  1. pExp-MsyB

    Plasmid
    #112570
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    112570-plasmid-map-sequence-id-255699

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  2. pEYFP-C1-NuMA

    Plasmid
    #28238
    Purpose
    Expresses human NuMA with N-terminal EYFP tag in mammalian cells
    Depositor
    Michael Mancini
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    28238-plasmid-map-sequence-id-373778

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  3. pExp-mCherry

    Plasmid
    #112569
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and mCherry
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112569-plasmid-map-sequence-id-217213

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  4. mTurquoise-Nesprin3-C-18

    Plasmid
    #55572
    Purpose
    Localization: Nuclear Membrane , Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Tags
    mTurquoise
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. mEos4b-Nup50-N-10

    Plasmid
    #57516
    Purpose
    Localization: Nuclear Pore, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    Nup50 (NUP50 Human)
    Tags
    mEos4b
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pExp-Bla

    Plasmid
    #112561
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-Bla and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112561-plasmid-map-sequence-id-217226

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  7. pExp-IF2

    Plasmid
    #112567
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-IF2 and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112567-plasmid-map-sequence-id-217215

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  9. mEos3.2-Nup50-N-10

    Plasmid
    #57479
    Purpose
    Localization: Nuclear Pore, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Nup50 (NUP50 Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Mutation
    BsaI site removed
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

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  11. pTH385-GST-TEV-SUP35

    Plasmid
    #29744
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    wild type
    Available Since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    29744-plasmid-map-sequence-id-13828

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  12. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  13. pExp-PpiB

    Plasmid
    #112572
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-PpiB and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112572-plasmid-map-sequence-id-217222

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  16. pcDNA3.1-hRIPK1-Flag-S320A

    Plasmid
    #112491
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S320A
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112491-plasmid-map-sequence-id-214534

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  17. pTH278-GST-TEV-SUP45

    Plasmid
    #29743
    Depositor
    Tobias von der Haar
    Insert
    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    wild type
    Available Since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    29743-plasmid-map-sequence-id-13827

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  19. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

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  20. pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]

    Plasmid
    #86454
    Purpose
    Nuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86454-plasmid-map-sequence-id-170900

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Showing: 8841 - 8860 of 16271 results