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pOPINTTGneoCD4

Plasmid

#252740

Purpose

Rat CD4 protein expression in mammalian cells

Depositor

Ray Owens

Article

Nettleship et al Methods Mol Biol. 2019;2025:33-49. doi:

Insert

CD4 (Cd4 Rat)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pOPINTTGneoFc

Plasmid

#252742

Purpose

Human IgG1 Fc protein expression in mammalian cells

Depositor

Ray Owens

Article

Nettleship et al Methods Mol Biol. 2019;2025:33-49. doi:

Insert

IgG1 Fc (IGHG1 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pHA-mMYO7A-Full

Plasmid

#251434

Purpose

Mammalian expression vector for expressing full-length mouse MYO7A with an N-terminal HA tag

Depositor

Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (Myo7a Mouse)

Tags

HA

Expression

Mammalian

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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mMYO7A-Full-pHA

Plasmid

#251435

Purpose

Mammalian expression vector for expressing full-length mouse MYO7A with a C-terminal HA tag

Depositor

Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (Myo7a Mouse)

Tags

HA

Expression

Mammalian

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pHalo2X-mMYO7A-Full

Plasmid

#251425

Purpose

Mammalian expression vector for expressing HaloTag-fused full-length mouse MYO7A

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (Myo7a Mouse)

Tags

HaloTag

Expression

Mammalian

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pIF1-S39E-GFP

Plasmid

#206937

Purpose

Plasmid expressing the mutant S39E of human IF1 fused in C-terminal with GFP

Depositor

José Cuezva

Article

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

S39E mutant of human IF1 (ATPase inhibitory factor 1)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 39 for a glutamic acid

Promoter

CMV

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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PAL-GFP

Plasmid

#221175

Purpose

Expresses the palmitolylation region of Arl13b and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13b (ARL13B )

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 10-428

Promoter

CMV promoter

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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mIFP-Talin1(1–2493)

Plasmid

#249169

Purpose

Mammalian expression vector of truncated talin1 lacks C-terminal dimerization domain (ΔDD)

Depositor

Pakorn Kanchanawong

Article

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

Talin1 (TLN1 Human)

Tags

mIFP

Expression

Mammalian

Mutation

Deleted amino acids 2494–2541

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E49TAG,G42H,K46H)-mCherry

Plasmid

#241292

Purpose

Mammalian expression of syntaxin-1(E49TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E49TAG,G42H,K46H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E53TAG,G42H,K46H)-mCherry

Plasmid

#241294

Purpose

Mammalian expression of syntaxin-1(E53TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E53TAG,G42H,K46H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(I233A,R210H,K84TAG)-mCherry

Plasmid

#241300

Purpose

Mammalian expression of syntaxin-1(I233A,K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

I233A,K84TAG,R210H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(K264A,K265A,K84TAG)-mCherry

Plasmid

#241301

Purpose

Mammalian expression of syntaxin-1(K264A,K265A,K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K264A,K265A,K84TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Rubicon-CGHL

Plasmid

#221658

Purpose

Rubicon mutated at four residues in the RH domain to disrupt Rab7 binding, tagged at the N-terminus with mCherry

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

mCherry

Expression

Mammalian

Mutation

C912G, C915G, H920L, C923G

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLOC-D133p53a

Plasmid

#241916

Purpose

Lentiviral vector driving a p53 isoform delta133p53alpha. Stable expression in cultured human cells and possibly cells of other species.

Depositor

Curtis Harris

Article

Ungerleider et al Neuroscience. 2022 Aug 21;498:190-202. d

Insert

Delta133p53alpha (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV TRE-Tight CheRiff-mKate2

Plasmid

#182493

Purpose

Expression of CheRiff channelrhodopsin linked to mKate2 from a Tet-inducible promoter

Depositor

Thomas Oertner

Article

Lamothe-Molina et al Nat Commun. 2022 Oct 26;13(1):6376. doi:

Insert

CheRiff (#51697 Adam Cohen Lab)

Use

AAV

Tags

mKate2 (Evrogen)

Expression

Mammalian

Promoter

TRE-Tight (Clontech)

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-HDAC1

Plasmid

#109209

Purpose

Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Synthetic, Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLG-mCCDC91 (198 a.a.)

Plasmid

#240069

Purpose

ITT reporter plasmid for cold stress

Depositor

Hodaka Fujii

Article

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

CCDC91 C-terminal 198 a.a. (Ccdc91 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPMEL-Nluc-hbb

Plasmid

#239862

Purpose

Template for in vitro transcription of secreted nanoluciferase, with signal peptide from PMEL, flanked by the human haemoglobin beta 5' and 3' UTRs.

Depositor

Catherine Jopling

Article

Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

Insert

Secreted nanoluciferase, with signal peptide from PMEL (gp100), flanked by human haemoglobin beta 5' and 3' UTRs

Use

Luciferase

Promoter

T7 (included as part of insert)

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-Hf-RfxCas13d-His

Plasmid

#234480

Purpose

Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

RfxCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Changed four alanine to valine in positions 134, …

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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LCV2 PQBP1 KO2

Plasmid

#217448

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting PQBP1 (PQBP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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