We narrowed to 2,693 results for: CCS
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Plasmid#191149PurposeExpression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun LuoDepositorInsertmCherry:s70587:Cre
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP
Plasmid#90008PurposeSplit-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBRC_double_attB_GFP_donor
Plasmid#169692Purposecargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)DepositorTypeEmpty backboneExpressionWormAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogTag-MBP
Plasmid#171773PurposeExpresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogTag-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK Tgfbr3-HA neo
Plasmid#58508PurposeLentiviral expression vector for an inducible HA-tagged mouse Tgfbr3DepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZS*12-MS2-GFP
Plasmid#26245DepositorInsertMS2-GFP
ExpressionBacterialMutationfive amino acid linker (Ser, Gly, Gly, Gly, Gly) …Available SinceNov. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO mouse shRNA 1 rictor
Plasmid#21341DepositorAvailable SinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir
Plasmid#90004PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]DepositorInsertsFKBP-linker-Myc-NBirA*
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
GART_pCSdest
Plasmid#53859DepositorAvailable SinceSept. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCG150_double_phiC31_attB
Plasmid#169693Purposecargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)DepositorTypeEmpty backboneExpressionWormAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
Plasmid#90009PurposeSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBPDepositorInsertsFKBP-linker-Myc-NBirA*
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B1
Plasmid#172842PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
AGPAT3_pCSdest
Plasmid#53928DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCH4 (denAsCas12a-KRAB lenti)
Plasmid#217331PurposeLentiviral expression of denAsCas12a-KRABDepositorInsertdenAsCas12a
UseCRISPR and LentiviralTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2MutationD908A/E174R/S542R/K548RPromoterUCOE-SFFVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_TUBA1B sgRNA / hSpCas9
Plasmid#172834PurposeMammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
DSCAM_pCSdest
Plasmid#53906DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX330_CALR sgRNA / hSpCas9
Plasmid#172838PurposeMammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CCT8_pCSdest
Plasmid#53795DepositorAvailable SinceSept. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lyn11-BirA*-HA
Plasmid#214474PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.DepositorInsertBirA(R118G)
UseLentiviral; GatewayTagsHA tag and Lyn11 (Plasma Membrane Localization Se…ExpressionMammalianMutationR118GPromoterEF-1aAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only