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pGEX4T1_GST-EGFP-ATG13_IDR_delta191-204 (205-517aa)

Plasmid

#223786

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 205-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa)

Plasmid

#223787

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

expresses aa 191-517, with 206-230 deleted

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGenLenti V5-NIX (E72A/L75A/D77A/E81A; deltaWIPI2)

Plasmid

#223789

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) (BNIP3L Human)

Use

Lentiviral

Tags

V5

Mutation

E72A/L75A/D77A/E81A; disrupts WIPI2 binding

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa)

Plasmid

#223758

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2 IDR (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Mutation

aa 364-425 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_mCherry-ATG13 HORMA (1-191aa)

Plasmid

#223759

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 HORMA (ATG13 Human)

Tags

mCherry

Expression

Mammalian

Mutation

aa 1-191 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR (191-517aa)

Plasmid

#223760

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 191-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

Plasmid

#223762

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

Plasmid

#223764

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

Thrombin cleavage-GST

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

Plasmid

#223765

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

EGFP-TEV-GST

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FKBP-mEGFP-WIPI1

Plasmid

#223767

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI1 (WIPI1 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FKBP-mEGFP-WIPI4

Plasmid

#223769

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI4 (WDR45 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (WT)

Plasmid

#223736

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Tags

EGFP-TEV-GST

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (WT)

Plasmid

#223737

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Tags

EGFP-TEV-GST

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a_GST-TEV-CK2alpha_CK2beta

Plasmid

#223740

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

CK2-alpha, CK2-beta (CSNK2A1 Human)

Tags

GST-TEV

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a_GST-TEV-CK2alpha'_CK2beta

Plasmid

#223741

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

CK2-alphaprime_CK2-beta (CSNK2A2 Human)

Tags

GST-TEV

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST

Plasmid

#223744

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BCL2L13 soluble part (BCL2L13 Human)

Tags

Thrombin cleavage-GST

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST

Plasmid

#223745

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BCL2L13 soluble part (BCL2L13 Human)

Tags

EGFP-TEV-GST

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1_GST-LC3A

Plasmid

#223726

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

LC3A (MAP1LC3A Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-120 only, with Glycine exposed

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-GBRP

Plasmid

#223728

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

GABARAP (GABARAP Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-GBRPL1

Plasmid

#223729

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

GABARAPL1 (GABARAPL1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge