Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 161,440 results for: Des

Showing: 8981 - 9000 of 161440 results

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pCEFL PRKACA HA WT

Plasmid

#249271

Purpose

Expression of PKA (PRKACA) wild type

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA R135W

Plasmid

#249274

Purpose

Expression of PKA R135W mutant (PRKACAR135W)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

R135W

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA E171K

Plasmid

#249275

Purpose

Expression of PKA E171K mutant (PRKACAE171K)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

E171K

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA T49I

Plasmid

#249272

Purpose

Expression of PKA T49I mutant (PRKACAT49I)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

T49I

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA E128K

Plasmid

#249273

Purpose

Expression of PKA T128K mutant (PRKACAE128K)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

E128K

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL_GPR161-HA_V129E

Plasmid

#249269

Purpose

Expression of GPR161 V129E mutant

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GPR161 (GPR161 Human)

Tags

HA

Expression

Mammalian

Mutation

V129E

Promoter

EF-1

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL_GPR161-HA

Plasmid

#249268

Purpose

Expression of GPR161 wild type

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GPR161 (GPR161 Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pEN243-KH217

Plasmid

#224550

Purpose

Cas9-2A-puro and sgRNA targeting the STOP codon mouse Car2

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9 and sgRNA targeting the STOP codon of mouse CAR2 (Car2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP14-SARS-CoV

Plasmid

#236424

Purpose

bacterial protein expression of NSP14

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP14 (ORF1ab SARS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP14-MERS-CoV

Plasmid

#236425

Purpose

bacterial protein expression of NSP14

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP14 (orf1ab MERS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP10-SARS-CoV-2

Plasmid

#236426

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (ORF1ab SARS-CoV-2)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP10-SARS-CoV

Plasmid

#236427

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (ORF1ab SARS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP10-MERS-CoV

Plasmid

#236428

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (orf1ab MERS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA E45A

Plasmid

#217438

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA E45A

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA Q63/67A

Plasmid

#217439

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA Q63A,Q67A

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA M66I

Plasmid

#217440

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA M66I

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HIV-1-GFP ΔEnv CA Q67H/N74D

Plasmid

#217441

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA Q67H,N74D

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.23-WIPI1promotor

Plasmid

#206840

Purpose

The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

Depositor

Tassula Proikas-Cezanne

Article

Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

Insert

WIPI1 promotor (partial)

Use

Luciferase

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET3a.H2B-K120C

Plasmid

#224706

Purpose

Expresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamers

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

H2B-K120C (H2B Human)

Expression

Bacterial

Mutation

K120C

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDual

Plasmid

#207883

Purpose

Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.

Depositor

David Edgell

Article

Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

Inserts

Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.

Use

Synthetic Biology

Expression

Bacterial

Mutation

Leucine 212 changed to proline, leucine 434 in th…

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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