We narrowed to 15,460 results for: pET

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Halotag-6xHp

Plasmid

#214414

Purpose

Lipid droplet localization of 6xHp of Spastin fused to Halotag

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (SPAST Human)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex2-rest1

Plasmid

#222143

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR F87E)

Plasmid

#242846

Purpose

Bacterial expression of TTR

Depositor

Peter Wright

Article

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine, F8…

Promoter

T7

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR)

Plasmid

#238123

Purpose

bacterial expression of human TTR

Depositor

Peter Wright

Article

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine

Promoter

T7

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH

Plasmid

#235294

Purpose

ComMAND closed-loop circuit regulating mRuby2 with 2 copies of microRNA target sites

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-HDAC3

Plasmid

#63676

Purpose

Mammalian expression of Flag tagged HDAC3

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

Insert

HDAC3 (Hdac3 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-sgCdkn2a/Cre

Plasmid

#89644

Purpose

Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Cdkn2a (Cdkn2a Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGal4AD-CIB1

Plasmid

#28245

Purpose

Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

Gal4AD (CIB1, GAL4 Budding Yeast)

Tags

CIB1

Expression

Yeast

Promoter

ADH1

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCMVTNT PINK1 N-myc

Plasmid

#13313

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen R119A F191A

Plasmid

#226574

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

R119A, F191A

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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psicheck2 PTEN 3'UTR

Plasmid

#50936

Purpose

reporter for PTEN RNAi

Depositor

Pier Pandolfi

Article

Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

Insert

PTEN 3'UTR (PTEN Human)

Use

RNAi

Tags

Renilla luciferase

Expression

Mammalian

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pT3TS-spCas9 ABE-Umax-ex1-rest1

Plasmid

#222140

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph20

Plasmid

#133024

Purpose

Expresses Xph20 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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FAB(mito-LD)

Plasmid

#214425

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-CAG-Flpe-ERT2

Plasmid

#68460

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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vGlut1 peptide- GST

Plasmid

#218677

Purpose

Expresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

vGlut (Slc17a6 Rat)

Tags

GST

Expression

Bacterial

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDule2-Acd82

Plasmid

#174100

Purpose

Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

Acridone 82 tRNA synthetase and tRNA

Expression

Bacterial

Mutation

N311S, C313G, V366A, W382T

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MFSD8-Puro

Plasmid

#239954

Purpose

Lentiviral vector to generate MFSD8 stable expressing cell line under CMV promoter

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD8

Use

Lentiviral

Tags

myc-flag

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pN565

Plasmid

#49990

Purpose

expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

T7 RNAP*

Use

Synthetic Biology

Tags

umuD degradation tag

Expression

Bacterial

Mutation

R632S

Promoter

pTac-symmetric

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pBJ5 hBRG1

Plasmid

#17873

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg (SMARCA4 Human)

Expression

Mammalian

Mutation

R1608Q

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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