167,910 results

1. FH-Tet2-pEF

   Plasmid

   #41710

   Purpose

   This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

   Insert

   tet methylcytosine dioxygenase 2 (Tet2 Mouse)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV BiP-Myc-KDEL-wt

   Plasmid

   #27164

   Purpose

   Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMRXIP GFP-Stx7

   Plasmid

   #45921

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   syntaxin 7 (STX7 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Promoter

   CMV

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pDONR223-GCK

   Plasmid

   #23750

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   GCK (GCK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMo130-TelR

   Plasmid

   #50799

   Purpose

   Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

   Depositor

   Kim Lee Chua

   Article

   Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

   Insert

   tellurite-resistance cassette
   

   Use

   Suicide vector

   Promoter

   PCs12

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.GFAP.Cre.WPRE.hGH

   Plasmid

   #105550

   Purpose

   AAV expression of Cre from GFAP promoter

   Depositor

   James M. Wilson

   Has Service

   AAV PHP.eB and AAV5

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCherry-NLS-mCherry

   Plasmid

   #231890

   Purpose

   mCherry-tagged nuclear rupture marker

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   mCherry-SV40 NLS-mCherry
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Kras-Src FRET biosensor

   Plasmid

   #78302

   Purpose

   FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

   Insert

   Src biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-NFAT1(4-460)-GFP

   Plasmid

   #11107

   Purpose

   Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

   Depositor

   Anjana Rao

   Article

   Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

   Insert

   NFAT1 (Nfatc2 Mouse)
   

   Tags

   3x HA and GFP

   Expression

   Mammalian

   Mutation

   Contains amino acids 4-460 of NFAT1; L78P compare…

   Promoter

   CMV

   Available Since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3.1_Zeo_TetO_FLAG-AT1R

    Plasmid

    #222856

    Purpose

    Mammalian expression plasmid for FLAG-tagged AT1R

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT1R (AGTR1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG PSAM4-5HT3 HC IRES EGFP

    Plasmid

    #119740

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)
    

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F, R420Q, R424D, R428A

    Promoter

    CAG

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dCas9-5xGCN4-P2A-BFP

    Plasmid

    #184438

    Purpose

    dCas9 fused with 5 copies of GCN4 epitope

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    dCas9-5xGCN4
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SPYtag-GFPnanobody

    Plasmid

    #214837

    Purpose

    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

    Insert

    SPYtag-GFPnanobody
    

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmCherry-SopF

    Plasmid

    #135174

    Purpose

    Expresses an mCherry-SopF fusion in mammalian cells

    Depositor

    Leigh Knodler

    Article

    Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

    Insert

    SopF (STM1239 Salmonella enterica serovar Typhimurium)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-Puro-hGSDME

    Plasmid

    #154876

    Purpose

    Expresses hGSDME in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. H6GB1tevP53SS(1-393)

    Plasmid

    #200311

    Purpose

    Bacterial expression of human superstable p53 as His6GB1tev fusion

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFGAMS-EGFP

    Plasmid

    #99107

    Purpose

    Expresses human PFAS-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PFAS (PFAS Human)
    

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ClCry4 PHR

    Plasmid

    #160552

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 HR
    

    Tags

    Hexa his

    Expression

    Insect

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CD44P pGL3

    Plasmid

    #19122

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 promoter (CD44 Human)
    

    Use

    Non viral cloning vector

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge