195,728 results

1. pHIV-iRFP720-E2A-Luc

   Plasmid

   #104587

   Purpose

   Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

   Depositor

   Antonius Plagge

   Article

   Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

   Insert

   iRFP720-E2A-Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   bi-cistronic

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pProEx RalA(G23C) CL

   Plasmid

   #224263

   Purpose

   Protein purification of RalA (G23C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   RalA (RALA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G23C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-KDEL_flGalT-SBP-EGFP

   Plasmid

   #65272

   Purpose

   synchronize trafficking of GalT from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   galactosidase
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304_CdTag-HA_EmptyVector

   Plasmid

   #221466

   Purpose

   Gateway backbone for C-terminal dTag cloning

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

   Plasmid

   #218857

   Purpose

   Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hGCGR (GCGR Human)
   

   Use

   Tags

   QF fusion protein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Td-CBEmax

   Plasmid

   #196600

   Purpose

   Mammalian expression of Td-CBEmax

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Use

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CRISPR

   Plasmid

   #160047

   Purpose

   For cloning sgRNA to knockout a specific gene in piggybac plasmid

   Depositor

   Xiaojun Lian

   Article

   Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-Tet-On-ARNTL-shRNA1

   Plasmid

   #166913

   Purpose

   Lentivirus for inducible knockdown of human ARNTL(BMAL1)

   Depositor

   Roland Friedel

   Insert

   ARNTL shRNA (BMAL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gs(short)-CASE

   Plasmid

   #168124

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG1

   Expression

   Mammalian

   Mutation

   NLuc is inserted at N136/V137 within GNAS

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG1169 FIREmate-ECFP-Cb5

    Plasmid

    #205167

    Purpose

    Expresses FIREmate-ECFP-Cb5 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    FIREmate-ECFP-Cb5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ANO6-Plvx-mCherry-c1

    Plasmid

    #62554

    Purpose

    Full length mouse ANO6 tagged with mCherry on the C-terminus

    Depositor

    Renzhi Han

    Article

    Zhao et al PLoS One. 2014 Mar 24;9(3):e92749. doi:

    Insert

    mouse ANO6 (Ano6 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW-OSKM

    Plasmid

    #20328

    Purpose

    Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. hCdh1 (1-125)

    Plasmid

    #8508

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hCdh1 (1-125) (FZR1 Human)
    

    Use

    Retroviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    Cdh1 N-terminus. Dominant Negative mutant.

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pLenti-HIFR

    Plasmid

    #192946

    Purpose

    Lentiviral production of HIF-1alpha reporter

    Depositor

    Markus Covert

    Article

    Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

    Insert

    HIF-1alpha reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mCMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EBFP2-N1

    Plasmid

    #54595

    Purpose

    Localization: N1 Cloning Vector, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. AARS_C1_pNIC-Bio3

    Plasmid

    #153046

    Purpose

    Expression construct for producing Avi tagged Alanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    AARS (AARS Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hsp68-LacZ-Gibson

    Plasmid

    #170102

    Purpose

    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.

    Depositor

    Len Pennacchio

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Mouse Hsp68 minimal promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TL026_AAV_hSyn:SEP-GluA1

    Plasmid

    #192519

    Purpose

    Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

    Depositor

    Adam Cohen

    Article

    Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

    Insert

    SEP-GluA1
    

    Use

    AAV and Cre/Lox

    Tags

    super ecliptic phluorin

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-ConVERGD-eGFP-WPRE

    Plasmid

    #218749

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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