We narrowed to 29,112 results for: NOL

1. A&C-BEmax

   Plasmid

   #157947

   Purpose

   Mammalian expression of A&C-BEmax

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   A&C-BEmax
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-ABEmax-NRCH

   Plasmid

   #136923

   Purpose

   Expresses ABEmax-NRCH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   ABEmax-NRCH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

   Plasmid

   #164612

   Purpose

   Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid non-responsive sensor GRAB_eCBmut
   

   Expression

   Mammalian

   Mutation

   S383T, F177A

   Promoter

   CMV

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YEE-BE4max

   Plasmid

   #138157

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YEE)-nSpCas9-UGI-UGI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Synapsin sHRPa-NRX in FSW lentiviral vector

   Plasmid

   #73147

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Use

   Lentiviral

   Tags

   2x HA tag and Flag tag

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pv6_TAF3_2xPhd

   Plasmid

   #179399

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   TAF3_2xPhd
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAVS1-Puro CAG PSMA nanobody T-CAR

   Plasmid

   #171962

   Purpose

   Donor plasmid for PSMA nanobody T-CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Harris et al J Immunol Regen Med. 2023 May;20:100074.

   Insert

   Synthetic anti-PSMA nanobody CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L2092-GPA-FullBRET-Retrovirus-TD142

   Plasmid

   #222877

   Purpose

   Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-NanoLuc-CyOFP1
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pv6_CBX7_2xChromo

   Plasmid

   #179396

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_2xChromo
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   G113V in flexible linker

   Promoter

   CAGGS

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX458-DsRed2

    Plasmid

    #112219

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    DsRed2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    DsRed2 contains a silent nucleotide substitution …

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SaCas9 TadCBEd

    Plasmid

    #193845

    Purpose

    Express TadCBEd (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd with SaCas9
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mTurquoise2-Paxillin

    Plasmid

    #176107

    Purpose

    Focal Adhesion marker

    Depositor

    Dorus Gadella

    Article

    Arts et al Front Immunol. 2021 May 18;12:667213. do

    Insert

    Paxillin (PXN Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eSpCas9(1.1)

    Plasmid

    #138555

    Purpose

    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CBE-T1.52

    Plasmid

    #193285

    Purpose

    Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CBE-T1.52
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205319

    Purpose

    All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISTfu1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pY094

    Plasmid

    #84743

    Purpose

    Expresses huAsCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    GFP
    

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mNeonGreen-Paxillin

    Plasmid

    #176106

    Purpose

    Focal Adhesion marker

    Depositor

    Dorus Gadella

    Article

    Arts et al Front Immunol. 2021 May 18;12:667213. do

    Insert

    Paxillin (PXN Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. gCH29 (crCD81-1)

    Plasmid

    #217344

    Purpose

    crRNA targeting CD81

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crCD81-1 (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CABE-T3.155

    Plasmid

    #193270

    Purpose

    Expression of CABE-T3.155 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T3.155
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET41-MBP-t-A3A-t-His

    Plasmid

    #109231

    Purpose

    Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

    Depositor

    Rahul Kohli

    Article

    Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

    Insert

    human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge