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pET-Galectin-8 FL

Plasmid

#68805

Purpose

Express Galectin-8 FL protein in Bacterial cells

Depositor

Hyun Kyu Song

Publication

Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

Insert

Galectin-8 (LGALS8 Human)

Tags

N-terminal 6-histidine-tag

Expression

Bacterial

Mutation

No mutation

Promoter

T7

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-GBP6-10gly-VPminx4

Plasmid

#50796

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP6-VPminx4 activation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FUW-TetO-Nr5a1

Plasmid

#41081

Depositor

Rudolf Jaenisch

Publication

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Nr5a1 (Nr5a1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pDuet TDP-43 Q331K

Plasmid

#27465

Depositor

Aaron Gitler

Publication

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Rheb1 shRNA #2

Plasmid

#26626

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pME18S-3HA-mULK1

Plasmid

#22896

Depositor

Masaaki Muramatsu

Publication

Yan et al Biochem Biophys Res Commun. 1998 May 8.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Tags

3xHA

Expression

Mammalian

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3-miR17

Plasmid

#21113

Depositor

Joshua Mendell

Publication

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

miR-17 (MIR17 Human)

Expression

Mammalian

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CMV-H3.3-YFP

Plasmid

#8693

Depositor

Kami Ahmad

Publication

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3.3A (His3.3A Fly)

Tags

YFP

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p043 mTcf-4 B

Plasmid

#11031

Depositor

Ramesh Shivdasani

Publication

Lee et al J Biol Chem. 1999 Jan 15. 274(3):1566-72

Insert

Tcf-4 (Tcf7l2 Mouse)

Use

Xenopus, avian, and zebrafish expression

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Publication

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Promoter

NF-kappaB binding site

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:NICD-P2A-mRFP

Plasmid

#127594

Purpose

Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

Depositor

Longhou Fang

Publication

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Insert

NICD (notch1a Zebrafish)

Use

Zebrafish expression

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_WT-PolyA

Plasmid

#112284

Purpose

Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

WT human YAP1-2gamma

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmCherry-C1-mTrim21ΔRING-Box

Plasmid

#105517

Purpose

Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of RING domain (aa124-462)

Promoter

CMV

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCLIPf-H2B Control Plasmid

Plasmid

#101134

Purpose

Expression of CLIP-tagged Histone H2B in mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Histone H2B (H2BC11 Human)

Tags

CLIP-tag (CLIPf)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Publication

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pEGFP-C1-Nup35

Plasmid

#87342

Purpose

To express human Nup35 in mammalian cells

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

nucleoporin 35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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eSrtA(4S-9) pET29b

Plasmid

#75146

Purpose

reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

Depositor

David Liu

Publication

Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

reprogrammed sortase A variant

Tags

6x His

Expression

Bacterial

Mutation

N98D S102C A104V A118T F122A K134R F144L I182V E1…

Promoter

T7

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pT7-EGFP-C1-HsNot3

Plasmid

#37372

Depositor

Elisa Izaurralde

Publication

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot3 (CNOT3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Publication

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Gateway Cloning

Tags

HA and Myr

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Publication

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article