We narrowed to 29,112 results for: NOL

1. pAAV-hSyn-hM3D(Gq)::RAM-d2tTA

   Plasmid

   #189627

   Purpose

   Expresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   hM3D(Gq)
   d2tTA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hSyn and pRAM

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-sgRNA(lib)-puro

   Plasmid

   #119976

   Purpose

   Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Wensheng Wei

   Article

   Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXL001-TQM IL13 CAR

   Plasmid

   #154054

   Purpose

   Lentiviral expression of TQM IL13 CAR for CAR-T therapy

   Depositor

   Xiaojun Lian

   Article

   Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

   Insert

   TQM IL13 CAR
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pePEmax-pPuro

   Plasmid

   #215688

   Purpose

   Cloning vector for Uni-Vector prime editing system

   Depositor

   Kou-Juey Wu

   Article

   Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

   Insert

   PEmax
   

   Use

   Unspecified

   Promoter

   EFS promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-GRAB_OT1.0

   Plasmid

   #185384

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pv6_MBD1_MBD

   Plasmid

   #179404

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1_MBD
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3/er-(n2)oxStayGold(c4)

   Plasmid

   #185822

   Purpose

   A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   er-(n2)oxStayGold(c4)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. L2125-FRB-GPA-NLuc-CyOFP1

   Plasmid

   #222885

   Purpose

   Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc-10GS-CyOFP1
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dnase1L3 pIRES-DsRed

   Plasmid

   #98845

   Purpose

   Expresses Human Dnase1L3 in mammalian cells along with DsRed

   Depositor

   Peter Keyel

   Article

   Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

   Insert

   Dnase1L3 (DNASE1L3 Human)
   

   Tags

   IRES DsRed

   Expression

   Mammalian

   Mutation

   long isoform, wild type

   Promoter

   CMV

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICSL11059

    Plasmid

    #68263

    Purpose

    Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK395 (AAVS1 CMV-OsTIR1(F74A))

    Plasmid

    #159992

    Purpose

    Expression of OsTIR1(F74A) in human cells

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74A)
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pv6_CBX7_Chromo_TAF3_Phd

    Plasmid

    #179400

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo - TAF3_Phd
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNCS-CyOFP

    Plasmid

    #74278

    Purpose

    Constitutive expression of CyOFP in bacteria

    Depositor

    Michael Lin

    Article

    Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    CyOFP
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. CBE-T1.14

    Plasmid

    #193277

    Purpose

    Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CBE-T1.14
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-LMP1-IRES-mCherry

    Plasmid

    #220351

    Purpose

    Expresses EBV latent gene LMP1 in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO-GRAB_eCB2.0

    Plasmid

    #164606

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

    Plasmid

    #218513

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of the Plac_mrfp cassette

    Promoter

    PgyrA_ermBL-ermB'

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

    Plasmid

    #193465

    Purpose

    Dn29 Donor with promoter for mcherry and puro expression

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Dn29_attP
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNLF1W

    Plasmid

    #136404

    Purpose

    Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sc++

    Plasmid

    #155011

    Purpose

    Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

    Insert

    Sc++
    

    Expression

    Mammalian

    Mutation

    T1227K, Loop Substitution from S. anginosus

    Promoter

    EF1ɑ

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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