We narrowed to 9,809 results for: crispr plasmids

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KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

Plasmid

#121837

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

Plasmid

#121811

Purpose

pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB

Plasmid

#121823

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/KRAB (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs N-terminal sgRNA

Plasmid

#207093

Purpose

pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGCGGGACTCGGCGGCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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P4-TadCBEd

Plasmid

#225143

Purpose

Packaging plasmid for TadCBEd in v3b PE-eVLP architecture

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-TadCBEd

Expression

Mammalian

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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gag-TadCBEd

Plasmid

#225144

Purpose

Packaging plasmid for TadCBEd in v3 PE-eVLP architecture

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-TadCBEd

Expression

Mammalian

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_T2

Plasmid

#184864

Purpose

pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_T1

Plasmid

#184863

Purpose

pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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MDC1 KO sgRNA

Plasmid

#207103

Purpose

pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GGTGTAACGTGGAGCCAGTA

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAbaMCi-GFP-gfp_pJMP2768

Plasmid

#208889

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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53BP1 N-terminal sgRNA

Plasmid

#207091

Purpose

pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GGGGAGCAGATGGACCCTAC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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ATM N-terminal sgRNA

Plasmid

#207089

Purpose

pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATCATTAAGTACTAGACTCA

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pFTK056

Plasmid

#171328

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9-NLS

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK063

Plasmid

#171335

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m4) (for fusion)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK062

Plasmid

#171334

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m2) (for fusion)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK061

Plasmid

#171333

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9 (for fusion)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK057

Plasmid

#171329

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m4)-VPR-NLS

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-Fluc-DR

Plasmid

#242434

Purpose

Firefly Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the TRE3G promoter.

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

Firefly Luciferase

Use

CRISPR and Luciferase

Tags

CasRx Direct Repeat

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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