We narrowed to 11,785 results for: emb

Showing: 9101 - 9120 of 11785 results

Results per page:

Sort results by:

pL0_EF-act1_full

Plasmid

#202172

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 full terminator

Use

Synthetic Biology

Mutation

None

Available since

Sept. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_EF-act1_tr

Plasmid

#202171

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 truncated terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_CD-PT2A

Plasmid

#202138

Purpose

Level 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructs

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Dual 2A peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_BC-mito_sig_pep

Plasmid

#202134

Purpose

Level 0 plasmid containing a kozak consensus sequence and a mitochondria signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Mitochondria signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_BC-sec_sig_pep

Plasmid

#202133

Purpose

Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Secretion signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_BC-chl_sig_pep

Plasmid

#202131

Purpose

Level 0 plasmid containing a kozak consensus sequence and a chloroplast signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Chloroplast signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJL1-MBP-DQNAT

Plasmid

#198215

Purpose

pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

MBP_DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-TOPO-DrSmg6_F

Plasmid

#146279

Purpose

Bacterial Expression of DrSmg6

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrSmg6 (LOC100334020 Zebrafish)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-TOPO-DrUPF2_F

Plasmid

#146282

Purpose

Bacterial Expression of DrUPF2

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF2 (upf2 Zebrafish)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-ShadowY

Plasmid

#193555

Purpose

Expresses mEGFP-ShadowY in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

ShadowY

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-p3E-GLRX-roGFP2

Plasmid

#194288

Purpose

Multisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQF

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

Gateway Cloning

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SpVasa-P662K-GFP

Plasmid

#185591

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

P662K

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SpVasa-mCherry-LOV

Plasmid

#185585

Purpose

for photoconversion

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

mCherry-LOV

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pE5773

Plasmid

#170780

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Publication

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-Spe3-RfA-HA

Plasmid

#186374

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-Spe3-HA-RfA

Plasmid

#186373

Purpose

Destination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-Spe3-RfA

Plasmid

#186370

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SZ1-GST-mCherry-RGG

Plasmid

#176646

Purpose

SynZip1 linked surfactant protein that localizes to RGG condensate interface

Depositor

Daniel Hammer

Publication

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

SZ1-GST-mCherry-RGG

Expression

Bacterial

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE-mClover3-bElys

Plasmid

#171494

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Elys mRNA

Depositor

Melina Schuh

Publication

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

Elys (AHCTF1 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP1064

Plasmid

#167607

Purpose

Express ExbD D25N protein

Depositor

Kathleen Postle

Publication

Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

Insert

exbD D25N

Expression

Bacterial

Mutation

exbD with D25N mutation

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge