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PX855

Plasmid

#62887

Purpose

N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Mutation

Aspartic acide 10 to Alanine (D10A)

Promoter

CBh

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PX855

Plasmid

#62887

Purpose

N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Mutation

Aspartic acide 10 to Alanine (D10A)

Promoter

CBh

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pAC-BETAipi

Plasmid

#53277

Purpose

Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

idi

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAC-BETAipi

Plasmid

#53277

Purpose

Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

idi

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEF5-FRT-TagRFP-T-IFT88

Plasmid

#61684

Purpose

Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

Depositor

Maxence Nachury

Publication

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IFT88 (Ift88 Mouse)

Tags

TagRFP.T

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEF5-FRT-TagRFP-T-IFT88

Plasmid

#61684

Purpose

Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

Depositor

Maxence Nachury

Publication

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IFT88 (Ift88 Mouse)

Tags

TagRFP.T

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Publication

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Publication

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCEP4-hERG1a

Plasmid

#53052

Purpose

Expresses alpha subunit of hERG1a K channel in mammalian cells

Depositor

Michael Sanguinetti

Publication

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCEP4-hERG1a

Plasmid

#53052

Purpose

Expresses alpha subunit of hERG1a K channel in mammalian cells

Depositor

Michael Sanguinetti

Publication

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-parkin C431S

Plasmid

#45877

Depositor

Edward Fon

Publication

Trempe et al Science. 2013 May 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

C431S mutation (ubiquitin acceptor site)

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pEGFP-parkin C431S

Plasmid

#45877

Depositor

Edward Fon

Publication

Trempe et al Science. 2013 May 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

C431S mutation (ubiquitin acceptor site)

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Publication

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Tags

6His

Expression

Bacterial

Promoter

T5

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Publication

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Tags

6His

Expression

Bacterial

Promoter

T5

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M-ST1cas

Plasmid

#48669

Purpose

Mammalian S. thermophilus #1 Cas9 expression, human optimized

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
M-ST1cas

Plasmid

#48669

Purpose

Mammalian S. thermophilus #1 Cas9 expression, human optimized

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
MSCV-CMV-DsRed-IRES-EGFP-DEST

Plasmid

#41941

Depositor

Stephen Elledge

Publication

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
MSCV-CMV-DsRed-IRES-EGFP-DEST

Plasmid

#41941

Depositor

Stephen Elledge

Publication

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
pEGFP(N3)-PP1beta

Plasmid

#44223

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Publication

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PP1B (PPP1CB Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pEGFP(N3)-PP1beta

Plasmid

#44223

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Publication

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PP1B (PPP1CB Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge