167,910 results

1. pCBL101

   Plasmid

   #199722

   Purpose

   A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   NPTII
   

   Expression

   Plant

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRG205MX

   Plasmid

   #64535

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 FLAG FBXW7alpha

   Plasmid

   #236470

   Purpose

   transient overexpression of FBXW7alpha in mammalian cells

   Depositor

   Michele Pagano

   Article

   Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

   Insert

   FBXW7alpha (FBXW7 Human)
   

   Expression

   Mammalian

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSQT1601

   Plasmid

   #53369

   Purpose

   human codon optimized Csy4 and FokI-dCas9 expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   hCsy4-T2A-NLS-hFokI-dCas9-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRG206MX

   Plasmid

   #64536

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

   Viral Prep

   #154867-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ulrik Gether

   Article

   Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

   Promoter

   Human Synapsin-1

   Tags

   mCherry (Flp-dependent)

   Available Since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

7. Anti-6xHis [N144/14-rat IgG2a] - Chimeric

   Plasmid

   #227037

   Purpose

   Mammalian expression plasmid of anti-6xHis rat IgG2a R-mAb. Derived from hybridoma N144/14.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-6xHis recombinant mouse monoclonal antibody.
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCP FLAG B55 alpha

   Plasmid

   #13804

   Depositor

   William Hahn

   Article

   Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

   Insert

   PP2A B55 alpha Flag (PPP2R2A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. SETD2(SET)-Cas9

   Plasmid

   #186700

   Purpose

   Plasmid encoding SETD2-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   SETD2(SET)-Cas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207180

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pODN-mNG-ANLN

    Plasmid

    #183834

    Purpose

    Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN homology arms with mNeonGreen-linker (ANLN Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_Ef1a_DIO_hTyrHA_minBack

    Plasmid

    #205286

    Purpose

    Induces NM-like pigment accumulation in catecholaminergic neurons

    Depositor

    Matthias Prigge

    Insert

    hTyrosinase
    

    Use

    AAV

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIZ-Flag6His-BmAgo3

    Plasmid

    #50559

    Purpose

    Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

    Depositor

    Yukihide Tomari

    Article

    Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

    Insert

    BmAgo3
    

    Tags

    6xHIS and Flag

    Expression

    Insect

    Promoter

    OpIE2

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. HMA-APOBEC1-Nb

    Plasmid

    #226012

    Purpose

    For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

    Depositor

    Eugene Yeo

    Article

    Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Tags

    His-MBP and antiRabbit-nanobody

    Expression

    Bacterial

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLacO-ISce1

    Plasmid

    #58505

    Purpose

    provides 256 Lac operator repeats adjacent to the ISce1 restriction site

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repeats adjacent to 6 copies of the ISce1 restriction site
    

    Expression

    Bacterial

    Promoter

    no promoter

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF3124

    Plasmid

    #144600

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

    Plasmid

    #219782

    Purpose

    CaST-IRES for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGT-B1

    Plasmid

    #122579

    Purpose

    MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. L1_lacZgRNA-Ck3

    Plasmid

    #136136

    Purpose

    L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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