164,782 results

1. pFB2E

   Plasmid

   #195288

   Purpose

   Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK EGFP 2A Puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CamKII-ArchT-GFP (PV2527) (AAV1)

   Viral Prep

   #99039-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Promoter

   CaMKII

   Tags

   GFP

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pTara:500

   Plasmid

   #60717

   Purpose

   Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-PHFF-sh-hBVRA

   Plasmid

   #100285

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Inserts

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   shRNA for human BVRA
   

   Use

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter and H1 promoter

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CAG-jGCaMP8m-WPRE (AAV Retrograde)

   Viral Prep

   #179255-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CAG

   Tags

   None

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

6. IDG_TMEM63B_OE_1

   Plasmid

   #161682

   Purpose

   Overexpresses TMEM63B

   Depositor

   Mike McManus

   Insert

   TMEM63B (TMEM63B Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPC1-muscle Amphiphysin 2

   Plasmid

   #22213

   Depositor

   Pietro De Camilli

   Article

   Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

   Insert

   Amphiphysin II (BIN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSC168

   Plasmid

   #216350

   Purpose

   xhNup35-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJYS3_ΔcrtYf

   Plasmid

   #85542

   Purpose

   Constitutive transcription of FnCpf1 and crRNA of crtYf

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRuby2-SiT-N-15

    Plasmid

    #55912

    Purpose

    Localization: Golgi TGN, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. gRNA_tdtomato_reporter_1_SA-2x

    Plasmid

    #79369

    Purpose

    Assays activity of transcriptional activators fused to SA Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOT_7-lenti-EFS-SS1-BBz-2A-puro

    Plasmid

    #217990

    Purpose

    Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Xue et al bioRxiv 2024.11.27.625752

    Insert

    SS1-BBz CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICE-EGFP-FLAG-PLIN3

    Plasmid

    #161918

    Purpose

    Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Perilipin-3 (PLIN3 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. BII-CPspaH2B

    Plasmid

    #133385

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV500 A-FOS

    Plasmid

    #33353

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    FOS (Fos Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative of c-Jun; heterodimerizes and i…

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG416GPD-Cerulean-YPT7

    Plasmid

    #18847

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT7 (YPT7 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-CAG-mRuby2

    Plasmid

    #99123

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Reciever_plasmid2

    Plasmid

    #140695

    Purpose

    Encodes quorum sensing (C4-HSL) inducible cyan (sfCFP) expression

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

    Insert

    sfCFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    engineered rhl promoter (responds to C4-HSL)

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBad-PhoCl2c

    Plasmid

    #164035

    Purpose

    Improved PhoCl variant with enhanced dissociation efficiency

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl2c
    

    Use

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBad promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223-EGFR

    Plasmid

    #23935

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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