178,426 results

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Publication

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMOS003-lenti-CMV-GCaMPer

Plasmid

#65227

Purpose

ER calcium sensor for lentivirus production

Depositor

Adam Cohen, Brandon Harvey

Publication

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

GCaMPer

Use

Lentiviral

Tags

circularly permuted GFP

Expression

Mammalian

Mutation

GCaMP3 (D324G, D360G, D397G, D435G)

Promoter

CMV

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRIPZ-hDDX5/17

Plasmid

#71307

Purpose

Inducible shRNA knockdown of both human DDX5 and DDX17 genes

Depositor

Allan Brasier

Publication

Yang et al Mol Cell Proteomics. 2015 Oct;14(10):270

Insert

DDX5/17 shRNA (DDX17 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

TetO

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-C3-PLK4-3xFLAG

Plasmid

#69837

Purpose

This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Publication

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmCherry-C1 actin-3XNLS P2A mCherry

Plasmid

#58475

Purpose

Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

Depositor

Dyche Mullins

Publication

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Inserts

beta actin (ACTB Human)
3XNLS
P2A cleavage site

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQE-60 Grx1-roGFP2-His

Plasmid

#64799

Purpose

Bacterial expression of His-tagged Grx1-roGFP2

Depositor

Tobias Dick

Publication

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Tags

His and roGFP2

Expression

Bacterial

Available since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSiM24-eGFP

Plasmid

#67883

Purpose

Plant Gene expression Vector pSIM24 containing eGFP reporter

Depositor

Indu Maiti

Publication

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Insert

eGFP

Expression

Plant

Promoter

M24

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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eGFPC1 FKBP

Plasmid

#67529

Purpose

Expresses GFP fused FKBP

Depositor

Stephen Tait

Publication

Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

Insert

FKBP

Tags

eGFP

Expression

Mammalian

Mutation

F36V

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TetO-FUW-NfiB

Plasmid

#64900

Purpose

Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Publication

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

NfiB (Nfib Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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BPK2139

Plasmid

#65776

Purpose

Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Promoter

CAG

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Mito-ABKAR

Plasmid

#61509

Purpose

Expression of AMPK biosensor targeted to the mitochondria

Depositor

Takanari Inoue, Jin Zhang

Publication

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Mitochondrial targeting signal (DAKAP1-30)

Expression

Mammalian

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
M_ST1n_VPR

Plasmid

#63799

Purpose

ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

Depositor

George Church

Publication

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

ST1-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pZDonor-Nm-Cas9-gRNA-hU6

Plasmid

#61366

Purpose

empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

Depositor

Charles Gersbach

Publication

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-Trastuzumab-IgM/κ

Plasmid

#61881

Purpose

Expresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)

Depositor

Andrew Beavil

Publication

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Mu heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-AKAR4-Kras

Plasmid

#61621

Purpose

Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Publication

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4-Kras

Tags

C-terminal targeting sequence from Kras, Cerulean…

Expression

Mammalian

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DR274-eGFP sgRNA

Plasmid

#61051

Purpose

for generation of a eGFP specific sgRNA from a T7 promoter

Depositor

Filippo Del Bene

Publication

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Insert

EGFP sgRNA

Use

CRISPR

Promoter

T7

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pICE-NLS-mCherry

Plasmid

#60364

Purpose

Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

Depositor

Sébastien Britton, Patrick Calsou

Publication

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

NLS-mCherry

Tags

NLS from SV40 T antigen and mCherry

Promoter

CMV-tet

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mEGFP-ER-5a

Plasmid

#56455

Purpose

Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

ER 5a (CALR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pmCTIP2-N174

Plasmid

#60858

Purpose

Expresses CTIP2 under the EF1a promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YFP NLS Beta-Actin G13R

Plasmid

#60615

Purpose

Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

Depositor

Primal de Lanerolle

Publication

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

G13R

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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