179,998 results
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Plasmid#106357PurposeEmpty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitationDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3XFLAG and HAExpressionMammalianPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pAAV.GfaABC1D.PI.Cre.SV40
Plasmid#105603PurposeAAV expression of Cre from GfaABC1D promoterDepositorInsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterGfaABC1DAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 (AAV5)
Viral prep#98930-AAV5PurposeReady-to-use AAV5 particles produced from pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 (#98930). In addition to the viral particles, you will also receive purified pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 plasmid DNA. GFAP-driven glutamate sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPAvailable sinceFeb. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-Arch-GFP (AAV1)
Viral prep#22222-AAV1PurposeReady-to-use AAV1 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA. CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPublicationPromoterCAGTagsGFP (Cre-dependent)Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-hphMX6
Plasmid#105156PurposeYeast gene targetingDepositorInsertmCherry-hphMX6
TagsmCherryExpressionBacterial and YeastAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET30b-7+-SrtA
Plasmid#105602Purposeexpressed 7+ sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7+ mutant
TagsHis tagExpressionBacterialPromoterT7Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV Retrograde)
Viral prep#55645-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
DNMT3A-dCas9
Plasmid#100090PurposeDNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDNMT3A (DNMT3A Human)
UseCRISPRTags3XFLag-NLS-DNMT3A-dCas9-NLSExpressionMammalianMutationincludes aa 602-912PromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAD-LyseR
Plasmid#99244PurposeFor autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-tTA
Plasmid#99118PurposeAn AAV genome that ubiquitously expresses tTA from the CAG promoterDepositorHas serviceAAV2InserttTA
UseAAVExpressionMammalianMutation*(see below)PromoterCAGAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry
Plasmid#85436PurposeLentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expressionDepositorInsertPembrolizumab and anti-CTLA4 scFv
UseLentiviralAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hUbC-dCas9-MQ1(Q147L)-EGFP
Plasmid#89793PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPR and LentiviralTags3*FLAG and T2A-EGFPExpressionMammalianMutationQ147L, CAA-->CTAPromoterhUbCAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SapTrap-SEC kit for C. elegans genome engineering
Plasmid kit#1000000109PurposeA collection of C. elegans plasmids used for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC), based on the SapTrap CRISPR/Cas Toolkit.DepositorApplicationGenome EditingVector typeWorm ExpressionEditing typeCRISPRAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-mCherry-HPos
Plasmid#87159PurposeFluorescent marker of lipid droplets (LDs)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHPos
TagsmCherryExpressionMammalianPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_DLL1_WT
Plasmid#82108PurposeGateway Donor vector containing DLL1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-gRNA-UbC-eGFP-P2A-Bsr
Plasmid#83925PurposeLentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastRDepositorInsertseGFP
Bsr
UseCRISPR and LentiviralTagsP2APromoterhUbCAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT3-EF1aH Yap S127A
Plasmid#86497PurposeMammalian expression of FLAG-tagged Yap containing S127A mutationDepositorInsertYes associated protein 1 (YAP1 Human)
TagsFLAGExpressionMammalianMutationS127APromoterEF1aAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSK9065
Plasmid#84345PurposeTightly controlled expression of mRFP fusion proteins in S. aureusDepositorTypeEmpty backboneTagsmRFPmarsExpressionBacterialPromoterPxyl/tetAvailable sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hGli3 flag3x
Plasmid#84921Purposemammalian expression of Gli3DepositorAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5E-gfap
Plasmid#82401PurposeGateway-compatible zebrafish gfap promoterDepositorInsertgfap promoter
Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by