We narrowed to 19,051 results for: Tec

1. pBK086

   Plasmid

   #183145

   Purpose

   pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

   Depositor

   Daan Swarts

   Article

   Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

   Insert

   MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniCGBE1-VRQR (pBM1173)

   Plasmid

   #140255

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_VRQR-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-2X-P2A-Puro

   Plasmid

   #110840

   Purpose

   Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-2X
   

   Use

   Lentiviral

   Mutation

   D10A and 2 tandem NLS sequences in the XTEN linker

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE80L-SpyCatcher-ELP-GFP

   Plasmid

   #69835

   Purpose

   Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyCatcher-ELP-GFP
   

   Tags

   6x His Tag, GFP, and TEV Tag

   Expression

   Bacterial

   Promoter

   T5 promoter/lac operator element

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpG-PPE

   Plasmid

   #170130

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

   Insert

   nCas9-SpG(H840A)-M-MLV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…

   Promoter

   maize Ubiquitin-1

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SECURE miniABEmax-V82G (pJUL1828)

   Plasmid

   #131313

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-HA-Flag-natT1R2 ECD-MHC

   Plasmid

   #113957

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG, Signal/leader sequence from influenza hemag…

   Expression

   Mammalian

   Mutation

   residues 22-568 fused to a transmembrane tether

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAPM-miR30-LRPPRC-ts1

   Plasmid

   #174268

   Purpose

   LRPPRC knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   LRPPRC shRNA (LRPPRC Human)
   

   Use

   Lentiviral and RNAi

   Promoter

   SFFV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAPM-miR30-LRPPRC-ts2

   Plasmid

   #174269

   Purpose

   LRPPRC knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   LRPPRC shRNA (LRPPRC Human)
   

   Use

   Lentiviral and RNAi

   Promoter

   SFFV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-xCas9RA-P2A-Puro

    Plasmid

    #110871

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. STK11 gRNA (BRDN0001147319)

    Plasmid

    #75914

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-FNLS-PGK-Puro

    Plasmid

    #110844

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. OCT4 GFP puro Donor 1

    Plasmid

    #22209

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-OCT-GFP-2APuro-PA (POU5F1 Human)
    

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMDC43-FIT2

    Plasmid

    #96993

    Purpose

    Express GFP-tagged mouse FIT2 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

    Insert

    MmFIT2 (Fitm2 Mouse)
    

    Tags

    GFP

    Expression

    Plant

    Mutation

    E2D (please see depositor comments below)

    Promoter

    35S

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGLO-GFP-3UAG

    Plasmid

    #82501

    Purpose

    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescence Protein with 3 UAG codons
    

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3, …

    Promoter

    AraBAD

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX020

    Plasmid

    #167146

    Purpose

    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal caffeoylpiruvate hydrolase, nnCPH
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid

    #113960

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-563

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX307-IBV-3CL

    Plasmid

    #160288

    Purpose

    Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRIM24 gRNA (BRDN0001146855)

    Plasmid

    #77886

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

    Plasmid

    #160624

    Purpose

    TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:MS2:VPR:Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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