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pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC

Plasmid

#148420

Purpose

Mammalian Expression of HsRck_296-472-10xmut

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-10xmut (DDX6 Human)

Expression

Mammalian

Mutation

Q309A, H312A, T316A, R320A, Q322A, N324A, R335A, …

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC

Plasmid

#148402

Purpose

Insect Expression of DmNot1_1674-1962-dsRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1_1674-1962-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-PML peptide

Plasmid

#175247

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Expression

Bacterial

Promoter

tac

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-RanGAP*

Plasmid

#175237

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2 gRNA resistant

Plasmid

#163408

Purpose

Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

gRNA-targeting sequences are substituted with nuc…

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-Ter

Plasmid

#170634

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Publication

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, Ter cassette

Expression

Bacterial

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-PRGRH-Nluc

Plasmid

#158608

Purpose

Plasmid expressing PRG(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-mas-p115RH-Nluc

Plasmid

#158607

Purpose

Plasmid expressing p115(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD/His-D-iLight

Plasmid

#170270

Purpose

Expression of iLight with 6xHis N-terminal tag in bacteria.

Depositor

Vladislav Verkhusha

Publication

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

iLight

Tags

6xHis tag

Expression

Bacterial

Mutation

See Comments

Promoter

araBAD

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1514

Plasmid

#155147

Purpose

Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

Depositor

Gira Bhabha, Damian Ekiert

Publication

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045B

Plasmid

#125004

Purpose

Expresses gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045D

Plasmid

#125006

Purpose

Expresses gRNAs targeting hedgehog and wingless

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

wg gRNA (wg Fly)

Expression

Insect

Promoter

u6:3

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986C

Plasmid

#125000

Purpose

Expresses dCas9-VPR driven by the bottleneck promoter

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

bottleneck

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986E

Plasmid

#125002

Purpose

Expresses dCas9-VP64 driven by the bottleneck promoter

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Expression

Insect

Promoter

bottleneck

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045A

Plasmid

#125003

Purpose

Expresses gRNAs targeting hid and eve

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050K (firefly luciferase)

Plasmid

#132422

Purpose

express arrays of gRNA targeting Firefly Luciferase under dU6-3 promoter

Depositor

Omar Akbari

Publication

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

firefly Luciferase gRNA array

Expression

Insect

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSP566

Plasmid

#139500

Purpose

Gene tagging vector to add mCherry Tag and membrane anchoring motif

Depositor

Serge Pelet

Publication

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

mCherry-CaaX

Expression

Yeast

Mutation

WT

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-S15

Plasmid

#127821

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-T136D-S15

Plasmid

#127824

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

T136D

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-S18

Plasmid

#127826

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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