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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,604 results for: LEA

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Showing: 9161 - 9180 of 16604 results

  2. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Thomas Hughes
    Insert
    Cre Shine
    Use
    Cre/Lox
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    37404-plasmid-map-sequence-id-114840

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  3. pAL-hCul1-NTD

    Plasmid
    #29518
    Depositor
    Ning Zheng
    Insert
    CUL1 (CUL1 Human)
    Tags
    Thrombin Cleavage Site
    Expression
    Bacterial
    Mutation
    N-terminal 415 aa
    Available Since
    May 26, 2011
    Availability
    Academic Institutions and Nonprofits only

  4. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Promoter
    Mouse Hsp68 minimal promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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  5. pEYFP-C1-NuMA

    Plasmid
    #28238
    Purpose
    Expresses human NuMA with N-terminal EYFP tag in mammalian cells
    Depositor
    Michael Mancini
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    28238-plasmid-map-sequence-id-373778

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  6. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pcDNA3.1-hRIPK1-Flag-delC

    Plasmid
    #112493
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of C terminus (AA290-671 deleted)
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112493-plasmid-map-sequence-id-214530

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  8. mTurquoise-Nesprin3-C-18

    Plasmid
    #55572
    Purpose
    Localization: Nuclear Membrane , Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Tags
    mTurquoise
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pmSod1-WT-N’His

    Plasmid
    #117701
    Purpose
    Expression of WT mouse SOD1 with N- terminal His tag
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117701-plasmid-map-sequence-id-227503

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  10. pET24a MDH_AQUAR

    Plasmid
    #204239
    Purpose
    Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_AQUAR
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204239-plasmid-map-sequence-id-413339

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  11. pET24a MDH_9CREN

    Plasmid
    #204243
    Purpose
    Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_9CREN
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204243-plasmid-map-sequence-id-413343

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  12. mEos4b-Nup50-N-10

    Plasmid
    #57516
    Purpose
    Localization: Nuclear Pore, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    Nup50 (NUP50 Human)
    Tags
    mEos4b
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pcDNA3.1-hRIPK1-Flag-delN

    Plasmid
    #112492
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of N terminus (AA1-274 deleted)
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112492-plasmid-map-sequence-id-214533

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  14. XClone_Cas9_KRAB_T2A_BFP

    Plasmid
    #141208
    Purpose
    Tunable and temporal expression control of dCas9
    Depositor
    Rory Johnson
    Insert
    dCas9
    Expression
    Mammalian
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    141208-plasmid-map-sequence-id-308770

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  15. pcDNA3.1-hRIPK1-Flag-delDD

    Plasmid
    #112494
    Purpose
    Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of DD domain (AA584-671 deleted)
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112494-plasmid-map-sequence-id-214528

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  17. Pf-GMPS

    Plasmid
    #36899
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pf-GMPS (PF10_0123 Plasmodium falciparum)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. PP-HSP90 (3O6O)

    Plasmid
    #28153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HSP90
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    N-terminal domain
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. RHEBL1A

    Plasmid
    #28156
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Ras homolog enriched in brain like 1 (RHEBL1 Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. Z-MATR3-4

    Plasmid
    #166558
    Purpose
    scFv of a human scaffold targeting Matrin-3. Antigen coverage aa 390-578 of 847
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166558-plasmid-map-sequence-id-329266

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Showing: 9161 - 9180 of 16604 results