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pBad33_ISAs26_reRNA

Plasmid

#205326

Purpose

Bacterial expression of ISAs26 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAs26 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAB168

Plasmid

#101671

Purpose

Opto-T7RNAP*(563-F1), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F1), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSB_syb25

Plasmid

#197992

Purpose

Expressed sybody 25 as a C-terminal myc-His-tagged construct

Depositor

Simon Newstead

Article

Parker et al Nat Struct Mol Biol. 2023 Jul 23. doi: 1

Insert

Sybody 25

Tags

Myc-His

Expression

Bacterial

Promoter

Arabinose

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSLICER

Plasmid

#197288

Purpose

Conjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transfer

Depositor

Bogumil Karas

Article

Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

Insert

I-SceI Endonuclease

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Ec2Avs2

Plasmid

#188790

Purpose

Avs2 from Escherichia coli NCTC10650

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec2Avs2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPTK009-3a-αAmylase-αMFΔ

Plasmid

#84968

Purpose

Type 3a, α-Amylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-Amylase followed by MFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pJB153

Plasmid

#92328

Purpose

Express mEos3.2 tagged ZapA in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

ZapA

Tags

mEos3.2

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGT-Ah5

Plasmid

#122572

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pICXNHG3

Plasmid

#49021

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

AOX1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pKEB31

Plasmid

#31224

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

ccdB and Cm resistance gene

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pSIBR4-Cre

Plasmid

#219435

Purpose

Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

intron 4-Cre

Expression

Bacterial

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPTK010-3a-Glucoamylase-αMFΔ

Plasmid

#84969

Purpose

Type 3a, Glucoamylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glucoamylase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBbE8c-RFP

Plasmid

#35269

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pNH051

Plasmid

#42157

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

GFP-RT-GFP

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pWS2247

Plasmid

#123026

Purpose

Tet promoter (TetO(x7)-pLEU2m)

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTet [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBbE6c-RFP

Plasmid

#35287

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pFRETgc-2in1-NC

Plasmid

#105121

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mCherry and mEGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWR

Plasmid

#112905

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHHYTK113

Plasmid

#200144

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

sfGFP1-10

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pITC

Plasmid

#192856

Purpose

Cloning and expression of transcription factor gene under control of naringenin inducible promoter

Depositor

Eveline Peeters

Article

Bernauw et al Methods Mol Biol. 2022;2516:113-141. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PfdeAR

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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