We narrowed to 18,537 results for: puro

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pX459-CTNNB1-S45

Plasmid

#164587

Purpose

Expresses a gRNA that overlaps the S45 codon of human CTNNB1

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 gRNA (CTNNB1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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p281 TIR1_HA_flox STOP_mH11

Plasmid

#164856

Purpose

Cre-inducible TIR1 expression vector for mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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TC1078-30

Plasmid

#164878

Purpose

30bp(mismatch17) cas13d gRNA for 1405 editing

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

gRNA for TC1405

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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PD13

Plasmid

#164530

Purpose

Expresses HA-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

HA and Ub noK

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-PD-L1 3SA/4NQ

Plasmid

#121491

Purpose

Expression of human PD-L1 3SA/4NQ with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRELA

Plasmid

#83944

Purpose

Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgRELA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone1

Plasmid

#162119

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgHK2_2

Plasmid

#163461

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting HK2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-AA

Plasmid

#160942

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Change Cys 287 Glu 288 into Ala Ala

Promoter

MSCV-LTR

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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PL652

Plasmid

#159456

Purpose

Donor empty vector with puromycin resistance gene expression cassette flanked by FRT

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLD1_2

Plasmid

#160792

Purpose

Suppress POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLD1_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P CISD1_1

Plasmid

#160773

Purpose

Suppress CISD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD1_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

Plasmid

#158542

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Tags

V5

Expression

Mammalian

Mutation

Amino acids 1-480, C-terminal deletion, F188D, W2…

Promoter

CMV

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL-C144A

Plasmid

#160285

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C144A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-MERS-CoV-3CL-C148A

Plasmid

#160283

Purpose

Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C148A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK19_IDG-K

Plasmid

#158175

Purpose

Gateway destination clone of CDK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK19 (CDK19 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-hcRed-V5-miniTurbo_IDG-K

Plasmid

#135237

Purpose

Gateway destination clone of hcRed (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

hcRed-V5-miniTurbo

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK17_IDG-K

Plasmid

#158174

Purpose

Gateway destination clone of CDK17 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK17 (CDK17 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-METTL14-WT

Plasmid

#141118

Purpose

Retroviral overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

METTL14 (METTL14 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_BFP2

Plasmid

#155097

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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