We narrowed to 14,380 results for: Cre
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Plasmid#158268PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHSV.Ollas.CMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HSV.Ollas.FLAG_NGFR
Plasmid#158270PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHSV.Ollas.FLAGMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FLAG.VSVg.C_NGFR
Plasmid#158260PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsFLAG.VSVg.CMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-SAE1
Plasmid#171012PurposeEctopic expression of SAE1DepositorInsertuntagged SAE1
UseLentiviralExpressionMammalianPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-nfBFP
Plasmid#171013PurposeEctopic expression of nonfluorescent BFPDepositorInsertuntagged nfBFP
UseLentiviralExpressionMammalianMutationBFP[H66A]PromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_Rat_cebp-alfa
Plasmid#140098PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_SPI1
Plasmid#140099PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p28BIOHTEV-LIC: nsp16_SARS-CoV-2
Plasmid#167846PurposeBacterial expression of the SARS-CoV-2 protein nsp16DepositorInsertnsp16
Tags6xHis and AviTagExpressionBacterialPromoterT7Available sinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-ACE2 receptor 19-615
Plasmid#167012PurposeGateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2DepositorAvailable sinceMarch 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFP-Rab8b in pCSDEST2
Plasmid#140881PurposeExpression of zebrafish GFP-tagged Rab8b in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab8b (rab8b Zebrafish)
TagsEGFPExpressionMammalianAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8a in pCSDEST2
Plasmid#140880PurposeExpression of zebrafish GFP-tagged Rab8a in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab8a (rab8a Zebrafish)
TagsEGFPExpressionMammalianAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniABEMax V82G wt
Plasmid#135362PurposeExpression of nSpyCas9 with N-terminal miniABEMax (V82G)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertminiABEMax nSpyCas9
UseCRISPRExpressionMammalianMutation(V82G)Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-ATP5O
Plasmid#139842PurposeLentiviral expression of human ATP5ODepositorInsertATP5O (ATP5PO Human)
UseLentiviralAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-actn2
Plasmid#109550PurposeMultisite gateway vector for 5' tagging with alpha actinin 2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha actinin 2
UseZebrafish plasmidsMutationPlease see Depositor CommentsAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD1b
Plasmid#109514PurposeMuscle specific zebrafish expression of mKate2 fused to EHD1bDepositorAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core
Plasmid#121839PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseGateway Cloning and Synthetic BiologyAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC194
Plasmid#118882Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
TagsSNAP tag, cleavable with TEV protease, HIS6, clea…ExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC231
Plasmid#118885Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease and mCitrine…ExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC254
Plasmid#118889Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease; mCherry, cl…ExpressionInsectPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only