Addgene

We narrowed to 31,076 results for: uros

Showing: 921 - 940 of 31076 results

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pLKO.1 shTGFBR3 puro

Plasmid

#58696

Purpose

Lentiviral shRNA vector for knockdown of human TGFBR3

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

shTGFBR3 (TGFBR3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
DNA-CARzeta-IRESpuro

Plasmid

#89345

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta

Use

Lentiviral

Tags

SNAPf tag

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pRS-puro shNEDD4L #2

Plasmid

#27017

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

shRNA NEDD4L #2 (NEDD4L Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 C311S

Plasmid

#40985

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C311S

Promoter

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pPBpuro-ErbB1(DEL1011)

Plasmid

#197366

Purpose

Encoding human ErbB1 mutant.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR mutant. (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

deleted amino acids 1011-1186

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-T7-hAIM2-EGFP

Plasmid

#51608

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Retroviral

Tags

EGFP and T7

Expression

Mammalian

Mutation

Promoter

MESV

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover

Plasmid

#124549

Purpose

Lentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2017 Apr 26;4(4):458-469.e5.

Insert

p65 promoter- p65 CDS-Clover

Use

Lentiviral

Tags

Clover

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 E321G

Plasmid

#40981

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

E321G

Promoter

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
tet-pLKO.puro_shHuR CDS

Plasmid

#110411

Purpose

TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

Depositor

Sandra Martha Gomes Dias

Insert

ELAVL1 (HuR)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1/TO (RNA PolIII)

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PB-EEA-g1-PGK-Puro

Plasmid

#102908

Purpose

PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-guide1-PGK-Puro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-mIR-31

Plasmid

#26088

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

miR-31 (MIR31 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2010

Availability

Academic Institutions and Nonprofits only
pMSCVpuro-Gcn5(D608A)

Plasmid

#63707

Purpose

retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

D608A mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
Neuroligin-3 GFP/pCMV

Plasmid

#197338

Purpose

Plasmid expressing an antigen targeting Neuroligin-3

Depositor

Thomas Südhof

Insert

Neuroligin3 (Nlgn3 Mouse)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-mPer2ER-pBABEpuro

Plasmid

#61430

Purpose

Express mPER2-ER* fusion protein

Depositor

Aziz Sancar

Article

Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

Insert

mPER2 (Per2 Mouse)

Use

Retroviral

Tags

Flag and mouse estrogen receptor ligand binding d…

Expression

Mutation

Promoter

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
Neuroplastin-CD4d3+4-bio

Plasmid

#73111

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Neuroplastin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Neuroplastin (NPTN Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN2

Plasmid

#125785

Purpose

DOX-inducible expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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TNF-SBP-mCherry_puromycin

Plasmid

#65285

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-puro

Plasmid

#208031

Purpose

Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

Plasmid

#81255

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc_SV40:PuroR

Plasmid

#183038

Purpose

Expresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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