Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,868 results for: pET

Showing: 9401 - 9420 of 9868 results

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pDonorRMCE-For-dSac-mTagBFP2

Plasmid

#253431

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dSac

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dSac Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dSac (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-dP2-mTagBFP2

Plasmid

#253432

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dP2

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dP2 Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dP2 (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-dP4-mTagBFP2

Plasmid

#253433

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dP4

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dP4 Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dP4 (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-dl1-mTagBFP2

Plasmid

#253434

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl1

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dl1 Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dL1 (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-dl2-mTagBFP2

Plasmid

#253435

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl2

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dl2 Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dl2 (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-dl3-mTagBFP2

Plasmid

#253436

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl3

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dl3 Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dl3 (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-dl6-mTagBFP2

Plasmid

#253437

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl6

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dl6 Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dl6 (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-dl8-mTagBFP2

Plasmid

#253438

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl8

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dl8 Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dl8 (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-Neutral-mTagBFP2-luciferase

Plasmid

#253441

Purpose

Modified donor plasmid for FLP/FRT RMCE in forward direction with longer reporter (mTagBFP2+luciferase) and Neutral promoter

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

mTagBFP2-t2a-luciferase

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

Neutral

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-dBx-mTagBFP2

Plasmid

#253429

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dBx

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dBx Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dBx (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorRMCE-For-dPst-mTagBFP2

Plasmid

#253430

Purpose

Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dPst

Depositor

Joanna Wysocka

Article

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

dPst Promoter

Use

Mouse Targeting; Flp/frt

Expression

Mammalian

Promoter

dPst (Ef1a mutant)

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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EKAR1

Plasmid

#254592

Purpose

ERK activity reporter with ECFP-YPet FRET pair

Depositor

Chuan-Hsiang Huang

Article

Wu et al iScience. 2025 Oct 11;28(11):113743. doi

Insert

EKAR1

Use

Lentiviral

Tags

ECFP and YPet

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pGTM-Sumo2-WN-NS2_3_TEE

Plasmid

#254047

Purpose

Expresses West Nile Virus NS3 and an NS2 fragment on a polyistronic message for E. coli expression

Depositor

Gaetano Montelione

Inserts

NS3
NS2

Tags

6xHis, Sumo

Expression

Bacterial

Promoter

Expressed as the second ORF in a polycistronic me…

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pGTM-Sumo2-Zika-NS2-NS3

Plasmid

#240390

Purpose

Expresses Zika virus protease in E. coli

Depositor

Gaetano Montelione

Insert

NS2B-NS3 protease

Tags

6xHis, Sumo

Expression

Bacterial

Mutation

NS2B-NS3 peptides fused by Gly-Ser linker

Promoter

T7 lac

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/T119M

Plasmid

#242712

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, T119M

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/F87A

Plasmid

#238120

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, F87A

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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M55

Plasmid

#242623

Purpose

Strep-Flag-MYCMutMBIV-His protein expression in bacteria

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-Flag-MYCMutMBIV-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Mutation

A310E,A311D,P312G,P313G,R315A,K316A,D317S

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAJC1

Plasmid

#240164

Purpose

Production of a thermostable enzyme, Pyrobaculum calidifontis manganese-dependent catalase, to be used in an educational laboratory exercise.

Depositor

Danielle Saunders

Article

Scott et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Inserts

katPc
Pc- kat

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/A25T

Plasmid

#238115

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S/S85C/A25T

Promoter

T7

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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TTR A120S

Plasmid

#238111

Purpose

bacterial expression of human TTR mutant

Depositor

Peter Wright

Article

Sun et al Protein Sci. 2024 Sep;33(9):e5101. doi:

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

A120S

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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