We narrowed to 47,369 results for: cha;

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pcDNA-Sub-Cas9

Plasmid

#227008

Purpose

Mammalian expression of Nuclease active S. uberis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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small ubiquitin-like modifier 3 (SUMO3)

Plasmid

#221053

Purpose

Expression of small ubiquitin-like modifier 3 (SUMO3) protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

SUMO 3 (SUMO3 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC1_pPromALB_enh5898_FireflyLuc

Plasmid

#220285

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 5898

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC2_pPromALB_enh29774_FireflyLuc

Plasmid

#220286

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 29774

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC3_pPromALB_enh50363_FireflyLuc

Plasmid

#220287

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50363

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC4_pPromALB_enh50358_FireflyLuc

Plasmid

#220288

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50358

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGYL17_pPromALB_enhALB_FireflyLuc

Plasmid

#220283

Purpose

Firefly luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

Alb Enhancer

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGYL18_pPromALB_enhALB_RenillaLuc

Plasmid

#220284

Purpose

Renilla luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pGL4.70 (Promega)

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

Alb Enhancer

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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RcSxph

Plasmid

#194018

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-HsTtyh1-TwinStrep-His

Plasmid

#211332

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Tweety homology protein 1

Use

Lentiviral

Tags

TwinStrep, 10xHis

Expression

Mammalian

Mutation

W417R

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR91

Plasmid

#203915

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

CRF1 plus flanking (CRF1 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR90

Plasmid

#203914

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

GSC2 plus flanking (GSC2 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR89

Plasmid

#203423

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

EGC3 plus flanking (EGC3 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Expression

Yeast

Promoter

pTEF1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Expression

Yeast

Promoter

pTPI1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-nCas9n

Plasmid

#217487

Purpose

Expression of nuclear nCas9n

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMS18

Plasmid

#215675

Purpose

Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS62

Plasmid

#215676

Purpose

Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Neo-LUC

Plasmid

#214368

Purpose

Expression of the firefly luciferase Protein

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

LUC

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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