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JL302: pMVP/Puro-DEST

Plasmid

#121856

Purpose

pMVP destination vector, empty expression vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127321

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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GHR1 X017_pECIA14

Plasmid

#114847

Purpose

Prey vector GHR1 X017_pECIA14 should be used with bait vector GHR1 X017_pECIA2.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G20940 (GHR1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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ERECTA C6_pECIA2

Plasmid

#115127

Purpose

Bait vector ERECTA C6_pECIA2 should be used with prey vector ERECTA C6_pECIA14.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G26330 (ER Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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HA-human-BNIP3 delta Exon2

Plasmid

#100782

Purpose

Mammalian expression of BNIP3 delta Exon2 splice variant

Depositor

Joseph Gordon

Publication

Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

Insert

human-BNIP3 delta Exon2 (BNIP3 Human)

Tags

HA

Expression

Mammalian

Mutation

Exon2 deletion

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pmEos2-Linker_PCNA-MutC

Plasmid

#98272

Purpose

for low level expression of mEos2-PCNA in mammalian cells

Depositor

Mike Heilemann

Publication

Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

Insert

PCNA (PCNA Human)

Tags

mEos2

Expression

Mammalian

Mutation

Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [D1_2] (GB1205)

Plasmid

#75406

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN aa452-526

Plasmid

#73381

Purpose

Expresses amino acids 452-526 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Publication

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 452-526 of XPLN (arhgef3) p…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa304-466 (PH)

Plasmid

#73380

Purpose

Expresses amino acids 304-466 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Publication

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 304-466 of XPLN (arhgef3) p…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa107-311

Plasmid

#73379

Purpose

Expresses amino acids 107-317 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Publication

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 107-317 of XPLN (arhgef3) p…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DIO-PBG-WPRE-hGH

Plasmid

#74287

Purpose

pAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Publication

Kim et al Neuron. 2015 Dec 16;88(6):1253-67. doi:

Insert

PBG (chimeric rabies Glycoprotein)

Use

AAV

Promoter

Ef1a

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pKG427

Plasmid

#69754

Purpose

Retroviral expression of DSN1

Depositor

Iain Cheeseman

Publication

Gascoigne et al J Cell Biol. 2013 Apr 1;201(1):23-32. do

Insert

DSN1 (DSN1 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

all K to R mutations

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only
2.3 hA33 luciferase

Plasmid

#68148

Purpose

luciferase reporter for hA33 promoter

Depositor

Joan Heath

Publication

Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

Insert

A33 (GPA33 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

starts at -2270 relative to ATG

Promoter

human GPA33 promoter

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83A

Plasmid

#58401

Purpose

Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 83 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24A

Plasmid

#58400

Purpose

Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 24 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
Human Mevalonate diphosphate decarboxylase (MDD)

Plasmid

#52431

Purpose

bacterial expression of human Mevalonate diphosphate decarboxylase (MDD)

Depositor

Henry Miziorko

Publication

Voynova et al Arch Biochem Biophys. 2008 Dec 1;480(1):

Insert

mevalonate diphosphate decarboxylase (MVD Human)

Tags

HIS

Expression

Bacterial

Promoter

T7

Available since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC

Plasmid

#47364

Purpose

Bmal fragment cloned with Venus tag and used for BiFC

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pmalc4x_V426F_TEV

Plasmid

#47702

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426F

Depositor

Raquel Lieberman

Publication

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with V426F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed V426F

Promoter

Ptac

Available since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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