We narrowed to 17,211 results for: pET

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pZF-BCB

Plasmid

#127471

Purpose

coding sequence for synthetic zinc finger ZF-BCB

Depositor

Sebastian Maerkl

Publication

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCB

Plasmid

#127471

Purpose

coding sequence for synthetic zinc finger ZF-BCB

Depositor

Sebastian Maerkl

Publication

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCB-GCN

Plasmid

#127476

Purpose

coding sequence for synthetic zinc finger ZF-BCB-GCN

Depositor

Sebastian Maerkl

Publication

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCB-GCN

Plasmid

#127476

Purpose

coding sequence for synthetic zinc finger ZF-BCB-GCN

Depositor

Sebastian Maerkl

Publication

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-A-005

Plasmid

#124412

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-A-005

Plasmid

#124412

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy9

Plasmid

#122004

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal StrepII with PreScission Cleavage site

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

StrepII

Expression

Bacterial

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy9

Plasmid

#122004

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal StrepII with PreScission Cleavage site

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

StrepII

Expression

Bacterial

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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FN(9-10)-I27-CC

Plasmid

#85394

Purpose

MTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

Depositor

Khalid Salaita

Publication

Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

Insert

Fibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon

Tags

6x His

Expression

Bacterial

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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FN(9-10)-I27-CC

Plasmid

#85394

Purpose

MTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

Depositor

Khalid Salaita

Publication

Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

Insert

Fibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon

Tags

6x His

Expression

Bacterial

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pR1G108CP20X

Plasmid

#85086

Purpose

Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Publication

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin

Tags

His6 tag

Expression

Bacterial

Mutation

G108C in PCNA1

Promoter

T7 promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pR1G108CP20X

Plasmid

#85086

Purpose

Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Publication

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin

Tags

His6 tag

Expression

Bacterial

Mutation

G108C in PCNA1

Promoter

T7 promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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DYRK2_HUMAN_D0

Plasmid

#79729

Purpose

This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK2 (DYRK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DYRK2_HUMAN_D0

Plasmid

#79729

Purpose

This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK2 (DYRK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHA3_HUMAN_D0

Plasmid

#79708

Purpose

This plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHA3 (EPHA3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DAPK3_HUMAN_D0

Plasmid

#79721

Purpose

This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK3 (DAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHA3_HUMAN_D0

Plasmid

#79708

Purpose

This plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHA3 (EPHA3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DAPK3_HUMAN_D0

Plasmid

#79721

Purpose

This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK3 (DAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CDKL1_HUMAN_D0

Plasmid

#79699

Purpose

This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDKL1 (CDKL1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CDKL1_HUMAN_D0

Plasmid

#79699

Purpose

This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDKL1 (CDKL1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only