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pK RagA-RagC(Q120L)

Plasmid

#99701

Purpose

Bacterial expression of RagA-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

Q120L

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG159-foxD

Plasmid

#99068

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea foxD

Depositor

Phillip Newmark

Publication

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

foxD in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup88-HindIII

Plasmid

#87329

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-XhoI

Plasmid

#87330

Purpose

To express shRNA against human Nup93

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE78

Plasmid

#80580

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [2_n-1] (GB1206)

Plasmid

#75407

Purpose

tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [2_n-1]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN (delta)N (aa1-117 deleted)

Plasmid

#73382

Purpose

Expresses myc tagged XPLN in mammalian cells with deletion of N-terminal amino acids 1-117

Depositor

Jie Chen

Publication

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Deleted N-terminal 1-117 aa from XPLN protein

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa1-125 (N125)

Plasmid

#73378

Purpose

Expression of the N-terminal region of XPLN (amino acids 1–125, designated N125)

Depositor

Jie Chen

Publication

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses N terminal amino acid 1-125 of XPLN (ar…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pLentiX2-PURO-shDIXDC1-Ms-57

Plasmid

#61232

Purpose

Lentivirus expressing shRNA to mouse DIXDC1

Depositor

Reuben Shaw

Publication

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

Dixdc1 (Dixdc1 Mouse)

Use

Lentiviral

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83,88,104A

Plasmid

#58405

Purpose

Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysines 83, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pBabe-Neuroserpin oloop

Plasmid

#58260

Purpose

Retroviral vector for expressing mutated Neuroserpin in mammalian cells

Depositor

Joan Massague

Publication

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Mutant of neuroserpin lacking five residues from …

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC I317D

Plasmid

#47367

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, I317D

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
BC-GPRC

Plasmid

#44199

Depositor

Lei Xu

Insert

human GPR56 C-terminal fragment (ADGRG1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

C-terminal fragment of GPR56 starting at amino a…

Promoter

LTR

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-LOC389599

Plasmid

#23361

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC389599 (STRADBP1 Human)

Use

Gateway Cloning

Mutation

C102T, several MMs

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDL12c

Plasmid

#17835

Depositor

Jay Keasling

Publication

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

gpdA promoter, lacZ, argB_1626,

Use

Synthetic Biology; Aspergillus nidulans expression

Mutation

The argB_1626 mutation consists of two sequential…

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 myc Caronte (CT#612)

Plasmid

#13861

Depositor

Cliff Tabin

Publication

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Tags

myc

Expression

Mammalian

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pHLsec hSOD1

Plasmid

#232481

Purpose

vector for transient transfection expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

Depositor

Lukas Trantirek

Publication

Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

Insert

Homo sapiens superoxide dismutase 1 (SOD1 Human)

Tags

DYKDDDDK (FLAG-tag)

Expression

Mammalian

Promoter

CAG

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgMlkl_#2-puro

Plasmid

#231980

Purpose

Knockout mouse Mlkl

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgMlkl_#1-puro

Plasmid

#231979

Purpose

Knockout mouse Mlkl

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK2-K82H-CR

Plasmid

#229425

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Publication

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK2-K82H (MARK2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Kinase-dead mutant K82H; silent mutation CRISPR r…

Promoter

EFS

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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