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We narrowed to 1,070 results for: gibson assembly

Showing: 941 - 960 of 1070 results

  1. pHRSV_CHARMS-3xA

    Plasmid
    #233926
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-3xA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233926-plasmid-map-sequence-id-469198

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  2. pHRSV_CHARMS-5x

    Plasmid
    #233927
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifs
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-5x
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233927-plasmid-map-sequence-id-469202

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  3. pHRSV_CHARMS-5xA

    Plasmid
    #233928
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5xA which contains only 5 TRAF6 binding motifs with a alanine mutation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-5xA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233928-plasmid-map-sequence-id-468363

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  4. pHRSV_CHARMS-S34Y

    Plasmid
    #233929
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-S34Y which contains a mutation in the death domain that disrupt oligomer formation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-S34Y
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233929-plasmid-map-sequence-id-469194

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  5. pHRSV_CHARMS-11xTA

    Plasmid
    #233932
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 11 TA repeats in place of the death domain
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-11xTA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233932-plasmid-map-sequence-id-469197

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  7. pBD21_035

    Plasmid
    #109152
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    EGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV and pTet
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109152-plasmid-map-sequence-id-209236

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  8. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid
    #196974
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag
    Depositor
    Lukas Kapitein
    Article
    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    GST and mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196974-plasmid-map-sequence-id-388923

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  9. pX330-BbsI-PITCh

    Plasmid
    #127875
    Purpose
    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly
    Depositor
    Peter Kaiser
    Article
    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127875-plasmid-map-sequence-id-482883

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  14. plentiCRISPR-sgGOT1

    Plasmid
    #72874
    Purpose
    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    sgGOT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pNC-Y

    Plasmid
    #238998
    Purpose
    Node C carrying sgRNA-1 that can be used for the assembly of pEND-11Y or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Article
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sgRNA-1 downstream of a weak constitutive promoter and a binding site for sgRNA-2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    238998-plasmid-map-sequence-id-479360

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  16. pHW621

    Plasmid
    #229867
    Purpose
    Entry vector with cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR in slot 5 (for SapTrap assembly)
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229867-plasmid-map-sequence-id-479101

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  20. MZB164

    Plasmid
    #217842
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    HaloTag
    Use
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217842-plasmid-map-sequence-id-433000

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Showing: 941 - 960 of 1070 results