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pET29b-clbI S178A

Plasmid

#114159

Purpose

Expresses ClbI S178A

Depositor

Emily Balskus

Publication

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbI

Tags

6xHis and 6xHis-Thrombin site

Expression

Bacterial

Mutation

Mutated S178 to Ala

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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YCe1897 HC_Kan_C-KDEL_8a

Plasmid

#100681

Purpose

part designed to occupy position 8a of EMMA. Functional category: Signal peptide for subcellular targeting

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

C-KDEL

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pJ02B2Rm_EF

Plasmid

#66060

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Rm_AE

Plasmid

#66065

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Gm_AE

Plasmid

#66057

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Gm_EF

Plasmid

#66058

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Rm_AE

Plasmid

#66059

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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B0033m_BC

Plasmid

#66021

Purpose

MoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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B0034m_BC

Plasmid

#66022

Purpose

MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pIR195

Plasmid

#64810

Purpose

TCP4 expression in yeast

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

TCP4 (TCP4 Mustard Weed)

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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LI D-E 11GFP

Plasmid

#54430

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

FLAG tag + and short linker + superfolder GFP 11 domain

Use

Cloning vector

Available since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C N-NES KOZ a c1

Plasmid

#54444

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CPK17-N-terminal + NES for enhanced export

Use

Cloning vector

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D Cerulean noATG

Plasmid

#54439

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CFP Cerulean without Start codon

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D YFP noATG

Plasmid

#54437

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

YFP Venus without Start codon

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LRRK2_1867_2161_F1908A_F2079G

Plasmid

#40414

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; F1908A, F2079G

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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LRRK2_1867_2161_V2048S_F2079G

Plasmid

#40417

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; V2048S, F2079G

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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LRRK2_1867_2161_L1914N_V2048T

Plasmid

#40424

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; L1914N, V2048T

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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LRRK2_1867_2161_L1914A_L1976Y

Plasmid

#40431

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; L1914A, L1976Y

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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LRRK2_1867_2161_L1976Y_I2049Q

Plasmid

#40435

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; L1976Y, I2049Q

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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LRRK2_1867_2161_V1922D_V2048S

Plasmid

#40444

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; V1922D, V2048S

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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