We narrowed to 25,810 results for: grna

1. lenti-EGFR-L858R-nick sgRNA

   Plasmid

   #214097

   Purpose

   Lentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR L858R nicking sgRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-EGFR-T790M-nick sgRNA

   Plasmid

   #214098

   Purpose

   Lentiviral vector expressing nicking sgRNA to induce EGFR T790M mutation using PE3

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR T790M nicking sgRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

   Plasmid

   #85714

   Purpose

   Telomere-targeting Nme sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   telomere sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC1812_pCR8-gCasRx-CUG (control gRNA)

   Plasmid

   #118645

   Purpose

   Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gCasRx-CUG
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-sgRNA(MS2)-puro-barcode

   Plasmid

   #89493

   Purpose

   Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   sgRNA (with MS2 loop)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SMVP-Cas9N-U6-gRNA TdR

   Plasmid

   #80940

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. non-targeting control gRNA (BRDN0001148500)

   Plasmid

   #80260

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

   Plasmid

   #181789

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSaCas9-sgRNA-Tetra-com vector

   Plasmid

   #131227

   Purpose

   For expressing gRNA, whose Tetraloop is replaced with a com aptamer

   Depositor

   Baisong Lu

   Article

   Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

   Insert

   vector for com modified sgRNA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

    Plasmid

    #163179

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126698

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    sgRNA targeting human SCGB3A2 locus
    

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiU6-hMYOD1 gRNA_1-MS2-Puro

    Plasmid

    #192673

    Purpose

    Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNA #1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiU6-hASCL1 gRNA_1-MS2-Puro

    Plasmid

    #192670

    Purpose

    Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNA #1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKSB-618 U6-sgRNA2/3 GGAA AACA

    Plasmid

    #173674

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

    Plasmid

    #181790

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

    Plasmid

    #133339

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL4 sgRNA(MS2)-MS2-hA3a

    Plasmid

    #112130

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL3 sgRNA(MS2)-MS2-AID3f

    Plasmid

    #112129

    Purpose

    empty sgRNA cloning vector with MS2-AID3f

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AIDmono 3f mutant (AICDA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL2 sgRNA(MS2)-MS2-AID3c

    Plasmid

    #112128

    Purpose

    empty sgRNA cloning vector with MS2-AID3c

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AID mono 3c mutant (AICDA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

    Plasmid

    #118845

    Purpose

    Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    ptena and ptenb (ptena, ptenb Zebrafish)
    

    Use

    CRISPR; Tol2

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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