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pCI-MEG3d

Plasmid

#59423

Purpose

Expresses lncRNA MEG3 isoform d in mammalian cells.

Depositor

Anne Klibanski

Publication

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3d (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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mCherryS131C

Plasmid

#48732

Purpose

Expresses mCherryS131C in E. coli cells

Depositor

Bradley Olsen

Publication

Thomas et al ACS Nano. 2011 Jul 26;5(7):5697-707. doi

Insert

mCherryS131C

Tags

6xHis

Expression

Bacterial

Mutation

changed serine 131 to cysteine

Promoter

T5

Available since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pBS ColI alpha2

Plasmid

#19207

Depositor

Cliff Tabin

Publication

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 type I collagen (COL1A2 Chicken)

Expression

Bacterial

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
TTR C10S S85C

Plasmid

#184410

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Publication

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSPLp10

Plasmid

#170462

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Publication

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

New vector

Expression

Insect

Promoter

dHsp70

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherryC1 VAP-A

Plasmid

#226407

Purpose

Expression of human VAP-A fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-A (VAPA Human)

Tags

T7-Xpress tag and mCherry

Expression

Mammalian

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SMN2E7-Fluc-Nluc-reporter

Plasmid

#221005

Purpose

Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

Depositor

Benjamin Blencowe, Mikko Taipale

Publication

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

Fluc-SMN2E7-STOP-Nluc

Expression

Mammalian

Promoter

CMV

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-AcGFP1-ORP9-PH

Plasmid

#214266

Purpose

Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

Depositor

Shinya Tsukiji

Publication

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

AcGFP1-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCG-TPMV Hd32

Plasmid

#163963

Purpose

Expresses TPMV H glycoprotein in mammalian cells

Depositor

Jakob Reiser

Publication

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

TPMV H glycoprotein

Expression

Mammalian

Mutation

Amino acids 2 to 33 at N terminus deleted

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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GZnP2 in pcDNA3.1+

Plasmid

#161739

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Publication

Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

Insert

GZnP2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-150

Plasmid

#118564

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC3_8-eGFP

Plasmid

#107411

Purpose

This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Publication

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC3_8

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKP3915

Plasmid

#113335

Purpose

Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Publication

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Artificial Transcription Factor (McIsaac et al, 2013)

Expression

Yeast

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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HSC1-GiP

Plasmid

#58254

Purpose

This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

Depositor

James Ellis

Publication

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

Plasmid

#38262

Depositor

Robert Lefkowitz

Publication

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag and PIP5K Iα core kinase domain (residues 18…

Expression

Mammalian

Mutation

Arg 233, Arg237, and Lys251 converted to Glutamin…

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Beta

Plasmid

#177962

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)

Depositor

Melanie Ott

Publication

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )

Expression

Mammalian

Mutation

K417N, E484K, N501Y, D614G

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJOE6261.2

Plasmid

#135079

Purpose

P. putida KT2440 rescue construct

Depositor

Josef Altenbuchner

Publication

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

upp (upp Pseudomonas putida)

Expression

Bacterial

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_PSMD1_sgRNA_1

Plasmid

#155102

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

PSMD1_sgRNA_1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_AAVS1_sgRNA_4

Plasmid

#155101

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_AAVS1_sgRNA_3

Plasmid

#155086

Purpose

lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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