We narrowed to 15,995 results for: LEA

1. pOPINVL

   Plasmid

   #26040

   Depositor

   Ray Owens

   Article

   Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

   Insert

   u-LacZ
   

   Tags

   u-phosphatase secretion leader

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINVH

   Plasmid

   #26041

   Depositor

   Ray Owens

   Article

   Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

   Insert

   u-LacZ-KHHHHHH
   

   Tags

   KHHHHHH and u-phosphatase secretion leader

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAH-ER-bapaUGAc

   Plasmid

   #201674

   Purpose

   boronic acid- and peptide-assisted UDP-GlcNAc sensor in ER

   Depositor

   Huiwang Ai

   Article

   Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

   Insert

   ER-bapaUGAc
   

   Tags

   ER leading sequence and ER retention sequence

   Expression

   Mammalian

   Promoter

   CMV SP6

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CFL1 (CFL1A-c002)

   Plasmid

   #98237

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CFL1A (CFL1 Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00241:Cofilin_ADF 27-148

   Promoter

   T7

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. EBFP2-PCNA-19-SV40NLS-4

   Plasmid

   #55250

   Purpose

   Localization: Nucleus, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   3xSV40 NLS and EBFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. 6975 pPROEX-HTb-SIS1

   Plasmid

   #1233

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   sis1 (SIS1 Budding Yeast)
   

   Tags

   his tag (tev cleavable)

   Expression

   Bacterial

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

7. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69546

   Purpose

   Tet-On system expressing MyoD-2A-GFP-2xNLS.

   Depositor

   Charles Gersbach

   Inserts

   eGFP
   Myod1 (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Mutation

   Removed stop codon to fuse it to the 2A-eGFPnls i…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYEXNH3

   Plasmid

   #49024

   Purpose

   FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. XLone-Puro NFIL3 P2A eGFP NLS

   Plasmid

   #140026

   Purpose

   Tunable and temporal expression control of NFIL3 and nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC-CFP

    Plasmid

    #173074

    Purpose

    Expression vector for E. coli producing target protein with N-terminal CFP fusion

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Type

    Empty backbone

    Tags

    mCerulean

    Expression

    Bacterial

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEGFP-PCNA-19-SV40NLS-4

    Plasmid

    #56469

    Purpose

    Localization: Nucleus, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. CMVTO-mCherry-Kir2.1-HA-tevs-RXR

    Plasmid

    #179704

    Purpose

    TEVP-inducible mCherry-conjugated Kir2.1 RELEASE

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    Kir2.1
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA_DiCre2_60.F2

    Plasmid

    #60210

    Purpose

    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)

    Depositor

    Jean-Paul Herman

    Insert

    Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)
    

    Tags

    FKBP12(F36V) and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    FKBP12 has the F36V mutation; amino acids 60-343 …

    Promoter

    CMV

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-6His-SUMO-Nsp14

    Plasmid

    #154956

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTETSIX

    Plasmid

    #74104

    Purpose

    mCherry-encoding vector for markerless deletion mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Insert

    tetA(C)
    

    Use

    Conjugative bacterial vector

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBXC3GH

    Plasmid

    #47070

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICXNH3

    Plasmid

    #49020

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4133639

    Plasmid

    #152344

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. 6974 pPROEX-HTb-SSB1

    Plasmid

    #1232

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    ssb1 (SSB1 Budding Yeast)
    

    Tags

    his tag (tev cleavable)

    Expression

    Bacterial

    Available Since

    Aug. 9, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pGBW-m4134388

    Plasmid

    #152696

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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