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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,126 results for: LEA

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Showing: 9821 - 9840 of 12126 results

  1. TBXTA-c019

    Plasmid
    #139756
    Purpose
    For protein expression
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-A214
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139756-plasmid-map-sequence-id-296514

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  6. pSR357.6

    Plasmid
    #125104
    Purpose
    Expression of narL(1-126aa)-ydfI(121-213aa) chimera under PLtetO-1, output Promoter PydfJ115
    Depositor
    Jeffrey Tabor
    Article
    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d
    Inserts
    narL(REC)-ydfI(DBD)126
    sfgfp
    tetR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    G207W- Please see depositor comments
    Available Since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125104-plasmid-map-sequence-id-247753

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  7. pHBSTC159

    Plasmid
    #124353
    Purpose
    Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    NpuDnaBΔ283 (C1G) mini-intein
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Deletion of 283-residue endonuclease domain, C1G
    Promoter
    STC
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124353-plasmid-map-sequence-id-242353

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  8. pOCC171

    Plasmid
    #118888
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; SNAP tag
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118888-plasmid-map-sequence-id-232245

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  9. Myc-gamma1 (in pCAGGS)

    Plasmid
    #118039
    Purpose
    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG1 (Gabrg1 Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    118039-plasmid-map-sequence-id-228636

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  10. phSOD1-L126X-N’His

    Plasmid
    #117707
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    L126X
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117707-plasmid-map-sequence-id-227541

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  11. phSOD1-D101G-N’His

    Plasmid
    #117704
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D101G
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117704-plasmid-map-sequence-id-227520

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  12. STK10 (STK10A-c043)

    Plasmid
    #110255
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STK10 (STK10 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110255-plasmid-map-sequence-id-212331

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  13. LIMK2 (LIMK2A-c052)

    Plasmid
    #110264
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    LIMK2 (LIMK2 Human)
    Tags
    His-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110264-plasmid-map-sequence-id-212269

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Showing: 9821 - 9840 of 12126 results