Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,929 results for: grna

Clear keywords
Showing: 9861 - 9880 of 15929 results

  6. pAL-pyk_T

    Plasmid
    #74070
    Purpose
    pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pyk (T)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pAL-pck_T

    Plasmid
    #74068
    Purpose
    pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pck (T)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. PX459v3-SpCas9-HF1

    Plasmid
    #178801
    Purpose
    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178801-plasmid-map-sequence-id-354837

    Enlarge

  9. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid
    #201677
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette
    Depositor
    Timo Otonkoski
    Article
    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-
    Insert
    MIR302-3g-EEA-2g-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201677-plasmid-map-sequence-id-395330

    Enlarge

  10. pEE531

    Plasmid
    #149290
    Purpose
    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2
    Depositor
    Daniel Voytas
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    Expression
    Plant
    Promoter
    35S
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149290-plasmid-map-sequence-id-305881

    Enlarge

  11. pEE491

    Plasmid
    #149292
    Purpose
    T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2
    Depositor
    Daniel Voytas
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    Expression
    Plant
    Promoter
    35S
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149292-plasmid-map-sequence-id-305826

    Enlarge

  13. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid
    #86611
    Purpose
    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    86611-plasmid-map-sequence-id-168847

    Enlarge

  17. pEf1a-hifiDn29-dCas9-P2A-GFP

    Plasmid
    #247158
    Purpose
    Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Insert
    hifiDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247158-plasmid-map-sequence-id-491688

    Enlarge

  19. SiC-V2-Cas9G7

    Plasmid
    #133043
    Purpose
    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.
    Depositor
    Sriram Neelamegham
    Article
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    Tet repressor
    Use
    Lentiviral
    Tags
    Cerulean
    Available Since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    133043-plasmid-map-sequence-id-264424

    Enlarge

Showing: 9861 - 9880 of 15929 results