We narrowed to 962 results for: cas12a

1. pYPQ285 (Lb5Cas12a)

   Plasmid

   #138120

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   Lb5Cas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ286 (CMaCas12a)

   Plasmid

   #138122

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized CMaCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   CMaCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ287 (BsCas12a)

   Plasmid

   #138123

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   BsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

   Plasmid

   #192453

   Purpose

   intronized ttLbCas12a for in planta expression

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
   

   Use

   CRISPR and Synthetic Biology; Moclo compatible le…

   Mutation

   temperature-tolerant mutation (D156R)

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-UltraCas12a-6xNLS

   Plasmid

   #209023

   Purpose

   Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   UltraCas12a-6xNLS-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   M537R/F870L

   Promoter

   EF-1a

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIBR2.0-818-Cas12a-NT

   Plasmid

   #235168

   Purpose

   Expresses the SIBR2.0-818-Cas12a system and a NT crRNA

   Depositor

   Constantinos Patinios

   Article

   Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

   Insert

   SIBR2.0-818-Cas12a
   

   Expression

   Bacterial

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti-As-Cas12a-2xNLS

   Plasmid

   #155047

   Purpose

   Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   AsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS, Myc-tag and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCE048-SiT-Cas12a

   Plasmid

   #128124

   Purpose

   Express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-FnCas12a-TEV

   Plasmid

   #174658

   Purpose

   FnCas12a expression plasmid

   Depositor

   Jin Wang, Huimin Zhao

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   FnCas12a
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-huMb2Cas12a

    Plasmid

    #174676

    Purpose

    Expresses 6xHis-MBP tagged Mb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-opCas12a-6xNLS

    Plasmid

    #209022

    Purpose

    Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-MBP-huTsCas12a

    Plasmid

    #174687

    Purpose

    Expresses 6xHis-MBP tagged TsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huTsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis-MBP-huMb3Cas12a

    Plasmid

    #174677

    Purpose

    Expresses 6xHis-MBP tagged Mb3Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb3Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNS35 (multiAsCas12a piggyBac)

    Plasmid

    #217336

    Purpose

    piggyBac expression of multiAsCas12a

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6xHis-MBP-huBsCas12a

    Plasmid

    #174671

    Purpose

    Expresses 6xHis-MBP tagged BsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-dCas12a-ABE8e

    Plasmid

    #193645

    Purpose

    Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e, EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS638_Flex-Cas12a-CBE

    Plasmid

    #244845

    Purpose

    Mammalian expression of Flex-Cas12a cytosine base editor

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    Flex-dCas12a-CBE
    

    Use

    CRISPR

    Tags

    2xSV40 NLS-APOBEC3A and Nucleoplasmid NLS-UGI-SV4…

    Expression

    Mammalian

    Mutation

    G146R/R182V/D535G/S551F/D665N/E795Q/D832A

    Promoter

    CMV

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYL-kasOp-FnCas12a3

    Plasmid

    #177875

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized the FnCas12a mutant EP16
    

    Use

    CRISPR

    Promoter

    kasOp*

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNHEJ-Cas12a-recX

    Plasmid

    #158714

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recX
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Pmyc

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCold CL7-Cas12a

    Plasmid

    #124891

    Purpose

    obtaining full Cas12a RNP

    Depositor

    Yi Liu

    Article

    Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

    Insert

    Cas12a
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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