We narrowed to 3,175 results for: tef

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pAV0782

Plasmid

#133514

Purpose

Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX

Expression

Yeast

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pNX3b-FL3

Plasmid

#78365

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
hygMX6

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAV11K

Plasmid

#63184

Purpose

pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Publication

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-CaURA3

Plasmid

#44879

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Expression

Yeast

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAV0612

Plasmid

#133505

Purpose

Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-mIAA7-3xFLAG-HIS3MX6

Plasmid

#247614

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

3xFLAG

Expression

Yeast

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGR721 - SLD3p-TP-DNAP1.Badboy2

Plasmid

#222013

Purpose

Expresses the Badboy2 TP-DNAP1 variant under control of a weak promoter for reducing p1 copy number

Depositor

Chang Liu

Publication

Rix et al Science. 2024 Nov 8;386(6722):eadm9073.

Insert

TP-DNAP1 BadBoy2

Expression

Yeast

Promoter

SLD3p

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTY24

Plasmid

#83794

Purpose

Useful for tagging yeast proteins with C-terminal DsRed tag; G418 selection

Depositor

Eric Muller

Publication

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

DsRed

Tags

DsRed

Expression

Yeast

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pKV014

Plasmid

#64765

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to 3xT7-mRFP tag, Hygro selection

Depositor

Fred van Leeuwen

Publication

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pYSGDLuc

Plasmid

#236297

Purpose

Dual luciferase reporter for measuring recoding efficiencies in yeast

Depositor

Pasha Baranov

Publication

Fenton et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Expression

Yeast

Promoter

TEF1

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Km ymTurquoise2

Plasmid

#193958

Purpose

TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeast

Depositor

Bas Teusink

Insert

ymTurquoise2

Expression

Yeast

Promoter

TEF1

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pUPD-G418R (01-21)

Plasmid

#101703

Purpose

G418R in domestication vector

Depositor

Elena Caro Bernat

Publication

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[G418R (loxP-PAgTEF-kanMX-TAgTEF-loxP)]-CGCT

Use

Yeast expression

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDP0437

Plasmid

#221127

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX

Depositor

Allyson O’Donnell

Publication

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

pHluorin

Expression

Yeast

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Publication

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Expression

Yeast

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-KanMx

Plasmid

#122896

Purpose

ADE2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-A1-FLAG:hphMX4

Plasmid

#134429

Purpose

PCR-based C-terminal A1-FLAG tag

Depositor

Robert Tomko

Publication

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

A1-FLAG

Use

Pcr-based tagging in yeast

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAV0526

Plasmid

#133500

Purpose

Expression of sfGFP targeted to the nucleus with one NLS signals

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0478

Plasmid

#133498

Purpose

Expression of sfGFP targeted to the nucleus with two NLS signals

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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GTU-g

Plasmid

#81094

Purpose

Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

Depositor

Serge Pelet

Publication

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

sfGFP

Use

Cre/Lox

Tags

sfGFP

Expression

Yeast

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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p41Neo MagicFluor

Plasmid

#58564

Purpose

p41Neo carrying both mating type markers buffered by the Cyc1 terminator

Depositor

Leonid Kruglyak

Insert

Ste2p-Citrine, Cyc1 term, Ste3p-mCherry

Expression

Yeast

Available since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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