Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 63,066 results for: cloning

Clear keywords
Showing: 9981 - 10000 of 63066 results

  3. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Available since
    Oct. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles
    68709-plasmid-map-sequence-id-125636

    Enlarge

  4. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

    Enlarge

  5. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

    Enlarge

  6. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

    Enlarge

  7. pDONR221-NADAR

    Plasmid
    #255737
    Purpose
    For Gateway cloning of NADAR
    Depositor
    Karla Feijs
    Insert
    SCO5665 (SCO5665 streptomyces coelicolor)
    Use
    Cloning
    Mutation
    codon optimised
    Available since
    July 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    255737-plasmid-map-sequence-id-515714

    Enlarge

  8. pDIV068

    Plasmid
    #247961
    Purpose
    Cloning vector containing A-PgpdA-PacI-Nt.BbvcI cassette-TtrpC-B sequences compatible with the DIVERSIFY gene landing platform
    Depositor
    Uffe Mortensen
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    247961-plasmid-map-sequence-id-501715

    Enlarge

  9. plix403-V5-N-mEYFP

    Plasmid
    #223955
    Purpose
    Gateway cloning of genes of interest with an N-terminal V5-mEYFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    V5-mEYFP
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223955-plasmid-map-sequence-id-450839

    Enlarge

  12. pCWX-R4DESTR2-PGK-Tk:Sh

    Plasmid
    #114321
    Purpose
    Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Zeocin/Bleomycin resistance and Acyclovir conditional ablation
    Depositor
    Patrick Salmon
    Type
    Empty backbone
    Use
    Lentiviral
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114321-plasmid-map-sequence-id-219916

    Enlarge

  17. pJRA109

    Plasmid
    #209342
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mTagBFP2 fusion protein.
    Depositor
    Lucia Strader
    Publication
    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209342-plasmid-map-sequence-id-420463

    Enlarge

Showing: 9981 - 10000 of 63066 results