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pMXs-IP-EGFP-ULK2(K39T)

Plasmid

#38206

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
MTK678_002

Plasmid

#123923

Purpose

Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BAC-AmpR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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766_SMAD4 in pmiRGlo

Plasmid

#78130

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

SMAD4 (SMAD4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAG217 PDLIM5 3'UTR mut

Plasmid

#12047

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N18 3'UTR mut (PDLIM5 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pFL-V5-GW

Plasmid

#78857

Purpose

DULIP Prey Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pPA-RL-GW

Plasmid

#78855

Purpose

DULIP Bait Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Protein A-Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-B4

Plasmid

#31708

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-92a

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pVH72-Tier1-PhCMV-SEAP

Plasmid

#169560

Purpose

Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven human secreted placental alkaline phosphatase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_VEGFB

Plasmid

#183328

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFB gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFB

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_VEGFA

Plasmid

#183327

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFA

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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MKK4

Plasmid

#29579

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK4 (MAP2K4 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
MXS_PGK::NeoR-bGHpA

Plasmid

#62438

Purpose

MXS_chaining vector with PGK::NeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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SERA1-bio-His

Plasmid

#50812

Purpose

Expresses enzymatically monobiotinylated full length SERA1

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA1

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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MTK3a_026

Plasmid

#123778

Purpose

Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sadCAS9 (with NLS)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P01

Plasmid

#120012

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_026

Plasmid

#123741

Purpose

Encodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Flamindo2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_043

Plasmid

#123943

Purpose

Encodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - A1 Insulator - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPD385 VPR-ZF43(RAAA)

Plasmid

#138824

Purpose

Constitutive expression of VPR-ZF43(RAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(RAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A, R71A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_GART

Plasmid

#183296

Purpose

All-in-One CRISPRko system with a guide RNA that targets GART gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

GART

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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