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pPOTv6-blast-His:mNG:His

Plasmid

#201094

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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12.4kbVillin-ΔATG

Plasmid

#19358

Depositor

Deborah Gumucio

Article

Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

Insert

12.4kb Villin promoter

Expression

Bacterial

Mutation

No ATG - transgenes cloned downstream of the vill…

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pRRL-Vinculin-Venus

Plasmid

#111833

Purpose

Vinculin (chicken) tagged with VenusA206K at the C-terminus in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

Vinculin-Venus (VCL Chicken)

Use

Lentiviral

Tags

VenusA206K

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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VinculinTS-A50I

Plasmid

#111827

Purpose

Vinculin tension sensor with point mutation affecting ability to bind talin

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (A50I)

Expression

Mammalian

Mutation

changed alanine 50 to isoleucine

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-cPLA2-mEGFP

Plasmid

#184718

Purpose

Exogenous cPLA2WT

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

cPLA2-mEGFP

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

Plasmid

#60660

Purpose

Flp-dependent expression of mNaChBacMut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

FLEX-mNaChBacMut-T2A-tdTomato

Use

AAV

Mutation

E191K

Promoter

EF1α

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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ASAP2f

Plasmid

#83960

Purpose

Fluorescent reporter for voltage imaging

Depositor

Michael Lin

Article

Yang et al Cell. 2016 Jun 30;166(1):245-57. doi: 10

Insert

ASAP2f

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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APEX2-csChBP

Plasmid

#108876

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csChBP

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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LeGO-EBFP2

Plasmid

#85213

Purpose

Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

Depositor

Boris Fehse

Article

Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

Insert

EBFP2

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAGM9121_GFP11

Plasmid

#153508

Purpose

GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PEBP1-Halo

Plasmid

#175333

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PEBP1 (PEBP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.F102Y.Y137L

Plasmid

#112177

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Expression

Mammalian

Mutation

F102Y.Y137L

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-eNpHR3.0-mRuby2-ST

Plasmid

#109134

Purpose

Halorhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eNpHR3.0-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-3Ty:mNG:3Ty

Plasmid

#179800

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pS24·Pm-GFP

Plasmid

#122594

Purpose

Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

Pm promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pm

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmGFP

Plasmid

#186542

Purpose

LSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-4493NES(Booster-PKA)

Plasmid

#184711

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato

Plasmid

#60658

Purpose

Flp-dependent expression of mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Has Service

AAV8

Insert

FLEX-mNaChBac-T2A-tdTomato

Use

AAV

Promoter

EF1α

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGL2_176

Plasmid

#199108

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

p15A ORI plus NGS barcode 24, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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