We narrowed to 118,701 results for: MPI

1. pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

   Plasmid

   #146001

   Purpose

   Insect Expression of DmSmaug_1-762

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug_1-762 (smg Fly)
   

   Tags

   V5-6xHis

   Expression

   Insect

   Mutation

   NP_523987.1, 7 silent mutations compared to the s…

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mLIG3-BRCT

   Plasmid

   #159321

   Purpose

   Mammalian cell expression of mouse LIG3-BRCT

   Depositor

   Jianjun Zhao

   Insert

   mLIG3-BRCT (Lig3 Mouse)
   

   Expression

   Mammalian

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNSU299_RPL22A

   Plasmid

   #166079

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Promoter of RPL22A (RPL22A Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE-60NA-mScarlet-I

   Plasmid

   #160462

   Purpose

   Encodes the original mScarlet-I

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   mScarlet-I
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRNATU6.1 HDAC3 shRNA1

   Plasmid

   #112249

   Purpose

   HDAC3 KD

   Depositor

   Simone Di Giovanni

   Insert

   shRNA against mouse HDAC3 (Hdac3 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

   Plasmid

   #159907

   Purpose

   Mutagenesis of Netrin1

   Depositor

   Larry Zweifel

   Insert

   Netrin1 (Ntn1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-rUSP19ER-siRNAresistant

   Plasmid

   #155251

   Purpose

   for mammalian overexpression of the siRNA resistant ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (ER isoform) siRNA resistant (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAMTA2.1.0-gDNA

   Plasmid

   #132456

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   CAMTA2 (CAMTA2 Human)
   

   Use

   CRISPR

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T-1-3xVSVG

   Plasmid

   #129568

   Purpose

   Empty backbone for VSVG tag

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T-1-3xGluGlu

    Plasmid

    #129571

    Purpose

    Empty backbone for GluGlu tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMBD1.1.0-gDNA

    Plasmid

    #132444

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    MBD1 (MBD1 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. KSP_HNL

    Plasmid

    #129772

    Purpose

    Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays

    Depositor

    William Hancock

    Insert

    KSP (kif11.L Frog)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Truncation at 367

    Available Since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Unc104_K560GFP

    Plasmid

    #129773

    Purpose

    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged

    Depositor

    William Hancock

    Insert

    Unc104 (unc-104 Nematode)
    

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 361, fused to kinesin-1 starting Al…

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p64T-XBMP2

    Plasmid

    #117253

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

    Insert

    BMP2
    

    Use

    Xenopus expression

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-XTbx5

    Plasmid

    #117247

    Purpose

    mRNA synthesis in situ hyb probes

    Depositor

    Gerald Thomsen

    Article

    Horb et al Development. 1999 Apr;126(8):1739-51.

    Insert

    Tbx5 (tbx5-b Frog)
    

    Use

    Xenopus expression

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSP64T-BMP4

    Plasmid

    #117254

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

    Insert

    BMP4
    

    Use

    Xenopus expression

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBbi-Pur-LPAR

    Plasmid

    #125205

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide

    Depositor

    Eric Davis

    Insert

    Lyn-Phospho-Activity-Reporter, Empty
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-mTRAF3ip3∆TM-CFP

    Plasmid

    #125182

    Purpose

    Expresses mouse TRAF3IP3 ∆TM in mammalian cells

    Depositor

    Shao-Cong Sun

    Insert

    TRAF3ip3 (Traf3ip3 Mouse)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    K87R and S359P- See depositor comments below

    Promoter

    CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZSCAN18-donor

    Plasmid

    #113821

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZSCAN18 with GFP

    Depositor

    Kevin White

    Insert

    ZSCAN18 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN18 Human)
    

    Use

    CRISPR

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7-5iX

    Plasmid

    #106991

    Purpose

    T7 transcription template for 5iX

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    5iX
    

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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