We narrowed to 2,026 results for: AIP;

1. pLenti6.2-Renilla-ccdB

   Plasmid

   #87076

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

   Depositor

   Mikko Taipale, Susan Lindquist

   Article

   Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti6.2-ccdB-3xFLAG-V5

   Plasmid

   #87071

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

   Depositor

   Mikko Taipale, Susan Lindquist

   Article

   Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti6.2-3xFLAG-V5-ccdB

   Plasmid

   #87072

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

   Depositor

   Mikko Taipale, Susan Lindquist

   Article

   Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PTPN13_PDZ_3

   Plasmid

   #103900

   Purpose

   Protein expression and purification of PTPN13 PDZ3 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MAGI1_PDZ_2

   Plasmid

   #103899

   Purpose

   Protein expression and purification of MAGI1 PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MAGI1_PDZ_1

   Plasmid

   #103898

   Purpose

   Protein expression and purification of MAGI1 PDZ1 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MAGI1_PDZ_4

   Plasmid

   #103901

   Purpose

   Protein expression and purification of MAGI1 PDZ4 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103_ABI2-1/1-SV1/2

   Plasmid

   #91285

   Purpose

   Protein expression and purification of human SH3 domain construct ABI2-1/1-SV1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ABI2-1/1-SV1/2 (ABI2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti6.2-Nanoluc-ccdB

   Plasmid

   #87078

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Nanoluc luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-ccdB-Nanoluc

    Plasmid

    #87067

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti6.2-ccdB-Nanoluc

    Plasmid

    #87075

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-Nanoluc-ccdB

    Plasmid

    #87070

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-ccdB-Renilla

    Plasmid

    #87065

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-ccdB-Firefly

    Plasmid

    #87066

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Firefly luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Firefly-ccdB

    Plasmid

    #87069

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti6.2-ccdB-Firefly

    Plasmid

    #87074

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Firefly luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-Renilla-ccdB

    Plasmid

    #87068

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti6.2-Firefly-ccdB

    Plasmid

    #87077

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEN-mSmoA1

    Plasmid

    #37674

    Depositor

    Philip Beachy

    Article

    Taipale et al Nature. 2000 Aug 31;406(6799):1005-9.

    Insert

    Smo (Smo Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W539L

    Promoter

    CMV

    Available since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEN-mSmo

    Plasmid

    #37673

    Depositor

    Philip Beachy

    Article

    Taipale et al Nature. 2000 Aug 31;406(6799):1005-9.

    Insert

    Smo (Smo Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

21. CMV-dCasRx-ccdB

    Plasmid

    #221003

    Purpose

    Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EF1a-dCasRx-RBM25

    Plasmid

    #221001

    Purpose

    Transient expression of dCasRx-RBM25. EF1a promoter.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLX303-ZIM3-KRAB-dCas9

    Plasmid

    #154472

    Purpose

    Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLX301-ZIM3-KRAB-PYL1

    Plasmid

    #154761

    Purpose

    Expresses ZIM3 KRAB domain fused to PYL1

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    CMV

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. SMN2E7-Fluc-Nluc-reporter

    Plasmid

    #221005

    Purpose

    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    Fluc-SMN2E7-STOP-Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLX303-ABI1-dCas9

    Plasmid

    #154474

    Purpose

    Expresses ABI1 fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ABI1 (ABI1 Mustard Weed)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Includes ABI1 aa 126-423, D143A

    Promoter

    CMV

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid

    #221002

    Purpose

    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25-IRES-BleoR
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR221 SARS-CoV-2 S-frag5_nostop

    Plasmid

    #153177

    Purpose

    Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth, Mikko Taipale

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S-frag5
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge