-
Plasmid#162976PurposeGateway attB4/attB5 entry clone for mammalian expression using the human PGK promoterDepositorInserthuman pGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E21: attB4-PGKp>-attB1r
Plasmid#162921PurposeGateway attB4/attB1r entry clone for mammalian expression using the human PGK promoterDepositorInserthuman pGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W
Plasmid#163170PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tagDepositorInsertYAP1 (YAP1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W
Plasmid#163172PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tagDepositorInsertYAP1 (YAP1 Human)
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)
Plasmid#164916PurposeExpresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTRDepositorInsertGCN4(UTR)-ZEM(TF)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPGK1 (yeast)Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMX-mPGK-CD3-P2A-CD90.2
Plasmid#163337PurposeRetroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the mPGK promoterDepositorInsertCD3
UseRetroviralTagsExpressionMammalianMutationPromotermPGKAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato. PGK-puro
Plasmid#136264PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO2(NGC)-PGK-Neo
Plasmid#136893PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO2(NGC)
UseLentiviralTags2xNLSExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO2(NGT)-PGK-Neo
Plasmid#136892PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO2(NGT)
UseLentiviralTags2xNLSExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v4)-PGK-Puro-BFP
Plasmid#117143PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v4 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v5)-PGK-Puro-BFP
Plasmid#117144PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v5 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP
Plasmid#117140PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v1 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP
Plasmid#117141PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v2 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)
Plasmid#115429PurposeSelection for in-frame fusion expression constructsDepositorTypeEmpty backboneUseTagsT2A-SpHIS5 and λNExpressionYeastMutationPromoterPGK1Available sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-FKBP-Zte-Cit-tv-DHFR
Plasmid#116049PurposeReporter plasmid for measuring protease activityDepositorInsertFKBP-Zte-Cit-tv-DHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-FKBP-Zte-Cit-hc-DHFR
Plasmid#116047PurposeReporter plasmid for measuring protease activityDepositorInsertFKBP-Zte-Cit-hc-DHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Mbd3b-pA-pgk-bsd
Plasmid#74920PurposeMammalian expression of Mbd3bDepositorInsertMnd3b (Mbd3 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-aVent1-pgk-hph
Plasmid#74916PurposeMammalian expresion of aVent1DepositorInsertVent1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-aBsx-pgk-hph
Plasmid#74913PurposeMammalian expression of aBsxDepositorInsertBsx
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-zNanogHD-pgk-hph
Plasmid#74910PurposeMammalian expression of zNanog HomeodomainDepositorInsertNanog
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only