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pSB1C3-J23110-B0034-mRFP1_Yellow

Plasmid

#160443

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Yellow

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_Tet

Plasmid

#62422

Purpose

MXS_chaining vector with Tet

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline inducible promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMX_hTAF5

Plasmid

#44336

Depositor

Marc Timmers

Publication

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF5 (TAF5 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo

Plasmid

#239948

Purpose

Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2

Depositor

Jan Carette

Publication

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

Fc2-MFSD6(L3)

Expression

Bacterial and Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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tsPurple chromoprotein promoter-less

Plasmid

#118007

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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amilGFP chromoprotein promoter-less

Plasmid

#118006

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pBW414hrpL-cIgfp

Plasmid

#61439

Purpose

pSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Publication

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs34-CI-LVA-t-plam-30gfp-t

Tags

LVA

Expression

Bacterial

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR Age HsSAS-6 ST

Plasmid

#46382

Purpose

For expression of N-terminal fusions.

Depositor

Pierre Gonczy

Publication

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Gateway Cloning

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEBB-BT

Plasmid

#36295

Depositor

Ezra Burstein

Publication

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Tags

BT-tag (peptide sequence from P.freudenreichii tr…

Expression

Mammalian

Promoter

EF1alpha

Available since

June 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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(p)odr-3::GFP::EGL-4

Plasmid

#100897

Purpose

GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

Depositor

Noelle L'Etoile

Publication

Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

Insert

56-2741: odr-3 promoter

Expression

Worm

Promoter

odr-3

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh

Plasmid

#208277

Purpose

Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Publication

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh

Expression

Mammalian

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(4CNW)RS

Plasmid

#222871

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYZ146

Plasmid

#98406

Purpose

Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.leu1 (leu1 Fission Yeast)

Use

CRISPR

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ173

Plasmid

#98410

Purpose

Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)

Use

CRISPR

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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BcLOV-mCh-SOS

Plasmid

#208279

Purpose

Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Publication

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCh-SOS

Use

Lentiviral

Promoter

SFFV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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MAC-N-GFP

Plasmid

#172974

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Tags

MAC-tag

Expression

Bacterial and Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf8

Plasmid

#158377

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf8 (ORF8 )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf3a

Plasmid

#158372

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf3a (ORF3a )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP11

Plasmid

#158362

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP11 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP12

Plasmid

#158363

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP12 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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