We narrowed to 9,485 results for: Coli

1. pKD-SepRS-EFSep

   Plasmid

   #34623

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Inserts

   SepRS (MMP0688 Methanocaldococcuc maripaludis)
   EF-Sep (tufB E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Ptac and Ptac (from SepRS- operon like)

   Available since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-FAS

   Plasmid

   #49810

   Purpose

   Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   fatty acyl-ACP thioesterase
   hydroxydecanoyl-ACP dehydratase
   b-ketoacyl-ACP synthase III
   3-ketoacyl-ACP reductase
   enoyl-(acyl carrier protein) reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon-optmized. See supplemental information of …

   Promoter

   T7

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA7

   Plasmid

   #79967

   Purpose

   TM-MAGE strain

   Depositor

   Morten Sommer

   Article

   Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

   Inserts

   Lambda Red recombinase beta subunit
   DNA adenine methylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYihI-N+C-term K-R

   Plasmid

   #233093

   Purpose

   Expression of GST-YihI with N+C-term lysines (K) mutated to arginine (R)

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   GST-YihI with N+C-term lysines (K) mutated to arginine (R)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   GST-YihI with N+C-term lysines (K) mutated to arg…

   Promoter

   Available since

   May 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pRAR101

   Plasmid

   #185046

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with amino acids 2-8 deleted, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Amino acids 2-8 deleted of L31p coding region

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRAR111

   Plasmid

   #185047

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with last 8 amino acids deleted, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Last 8 amino acids deleted of L31p coding region

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRAR120

   Plasmid

   #185041

   Purpose

   J23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Only first 90 nt of rpmE coding present

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSMART-Ala2

   Plasmid

   #71326

   Purpose

   NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

   Depositor

   Michael Lynch

   Article

   Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

   Insert

   NADPH Alanine Dehydrogenase (ald Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   insulated waaHp from E. coli

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVR2

   Plasmid

   #84719

   Purpose

   E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   12 nt stretch lacking A's inserted before 5&…

   Promoter

   Available since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. His8:MBP-tev-Ala-eK-Flv

    Plasmid

    #83393

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Article

    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. His8:MBP-tev-Ile-eK-Flv

    Plasmid

    #83392

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Article

    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. His8:MBP-tev-Tyr-eK-Flv

    Plasmid

    #83391

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Article

    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKP968

    Plasmid

    #167653

    Purpose

    Express colicinB-His6, D17C, M261C

    Depositor

    Kathleen Postle

    Insert

    cba-his6 D17C, M261C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cba-his6 with D17C, M261C mutations

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. L5-LexAp65-L2

    Plasmid

    #41437

    Depositor

    Steve Stowers

    Insert

    LexAp65
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJOE6087.3

    Plasmid

    #135082

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Insert

    P-rhaP-BAD-eGFP-His tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. R4-LexAp65-R3

    Plasmid

    #41438

    Depositor

    Steve Stowers

    Insert

    LexAp65
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGLU_103

    Plasmid

    #225785

    Purpose

    This is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.

    Depositor

    Nili Ostrov

    Insert

    Ptn5_[Tn5]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGLU_85

    Plasmid

    #225800

    Purpose

    This is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.

    Depositor

    Nili Ostrov

    Insert

    PmarC9_[Himar]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKP996

    Plasmid

    #167654

    Purpose

    Express colicinB-His6, T18C, M261C

    Depositor

    Kathleen Postle

    Insert

    cba-his6 T18C, M261C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cba-his6 with T18C, M261C mutations

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKP995

    Plasmid

    #167655

    Purpose

    Express colicinB-His6, M19C, M261C

    Depositor

    Kathleen Postle

    Insert

    cba-his6 M19C, M261C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cba-his6 with M19C, M261C mutations

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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